All CHEK2 reagents are produced in house and quality controlled, including 1 CHEK2 Antibody, 30 CHEK2 Gene, 1 CHEK2 IPKit, 2 CHEK2 Lysate, 2 CHEK2 Protein, 2 CHEK2 qPCR. All CHEK2 reagents are ready to use.
Recombinant CHEK2 proteins are expressed by Baculovirus-Insect Cells with fusion tags as N-GST & His, N-GST.
CHEK2antibodies are validated with different applications, which are WB, IHC-P, IP.
CHEK2cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each CHEK2 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Expression host: Baculovirus-Insect Cells
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.