Mouse CHI3L1 / gp39 Protein (His Tag)

Chitinase 3-like 1 Protein Datasheet

CHI3L1 / gp39 Protein Product Information

• Synonym: AW208766, Brp39, Gp39
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CHI3L1 (Q61362 ) (Met 1-Ala 381) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

CHI3L1 / gp39 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Tyr 371
• Molecular Mass: The secreted recombinant mouse CHI3L1 comprises 371 amino acids and has a calculated molecular mass of 42.3 kDa. As a result of glycosylation, the apparent molecular mass of rmCHI3L1 is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CHI3L1 / gp39 protein

CHI3L1 / gp39 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CHI3L1 / gp39 Protein Description

Chitinase 3-like 1 (CHI3L1), also named Cartilage glycoprotein-39 (gp-39), is a secretory glycoprotein and member of the chitinase protein family. It is associated with late stages of monocyte to macrophage maturation. Human cartilage glycoprotein 39 (HC-gp39) is a 39-kDa glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Increased levels of HC-gp39 (CHI3L1) have been demonstrated in synovial fluids of patients with rheumatoid or osteoarthritis. It is differentially expressed at the site of chronic inflammation. CHI3L1 is one of the putative autoantigens in rheumatoid arthritis (RA). It is considered as a target of the immune response in RA. Besides, chinase 3-like 1 (CHI3L1) is an inducible molecule overexpressed in colonic epithelial cells (CECs) under inflammatory conditions.

References

1. Recklies AD. et al., 2002, Biochem J. 365 (1): 119-26.
2. Rehli M. et al., 1997, Genomics. 43 (2): 221-5.
3. Gijs FM. et al., 1997, Arthritis & Rheumatism. 40 (6): 1115-25.
4. Chen CC. et al., 2011, Clin Immunol. 140 (3): 268-75.