Mouse CHI3L1 Gene cDNA Clone / ORF Clone

CHI3L1 cDNA Clone Datasheet

CHI3L1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007695.3
• cDNA Size: 1170
• Gene Synonym: Gp39; Brp39; AW208766; Chi3l1
• Source: Mouse

CHI3L1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus chitinase 3-like 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CHI3L1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CHI3L1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CHI3L1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCACACCT CTACTGAAGC CAGGATGGGC ATGAGGGCGG CACTGACAGG CTTTGCGGTC
61       CTGATGCTGC TCCAGAGCTG CTCTGCGTAC AAGCTGGTCT GCTACTTCAC CAGCTGGTCC
121      CAGTACCGGG AAGGCGTTGG AAGCTTCTTA CCAGACGCCA TCCAACCTTT CCTGTGCACC
181      CACATCATCT ACAGCTTTGC CAACATCAGC AGCGACAACA TGCTTAGCAC ATGGGAGTGG
241      AATGACGAGT CGAACTATGA CAAGCTGAAT AAACTGAAGA CCAGAAACAC CAACCTGAAG
301      ACCCTCCTGT CTGTTGGAGG GTGGAAATTT GGCGAAAAAA GATTTTCCGA GATTGCCTCC
361      AACACTGAGA GACGCACTGC TTTCGTCCGG TCGGTAGCCC CGTTCCTGCG TTCTTATGGC
421      TTTGATGGGC TGGATCTCGC CTGGCTCTAC CCTCGCTTAA GAGACAAGCA GTATTTCTCC
481      ACCCTGATCA AGGAACTGAA TGCGGAATTC ACAAAGGAGG TCCAGCCAGG CAGAGAGAAA
541      CTCCTGCTCA GCGCAGCTTT GTCAGCAGGA AAGGTGGCCA TTGACACTGG CTATGACATC
601      GCCCAGATAG CCCAACACCT GGATTTTATC AATCTCATGA CCTACGATTT CCATGGAGTC
661      TGGCGCCAAA TCACAGGCCA TCACAGCCCC CTCTTCCAAG GCCAGAAGGA CACTAGGTTT
721      GACAGATACA GCAATGTGAA CTATGCCGTG CAGTACATGA TACGTCTGGG AGCCCAGGCC
781      AGCAAGCTAC TGATGGGCAT CCCCACCTTT GGGAAGAGCT TCACTCTGGC ATCTTCTGAA
841      AATCAGTTGG GAGCTCCAAT CTCAGGGGAA GGATTACCAG GCCGGTTCAC CAAGGAGGCA
901      GGGACCCTGG CCTACTACGA GATATGCGAC TTCCTCAAAG GAGCTGAAGT ACATCGACTC
961      TCCAACGAGA AGGTTCCCTT CGCTACCAAG GGCAACCAGT GGGTGGGGTA TGAGGACAAG
1021     GAGAGTGTCA AAAACAAGGT TGGGTTCCTG AAGGAGAAGA AGCTGGCAGG AGCCATGGTG
1081     TGGGCACTGG ATTTGGATGA TTTCCAGGGC ACCTGTCAGC CGAAGGAATT CTTCCCGCTC
1141     ACCAACGCCA TCAAGGATGC CCTGGCTTAG

 

Quality Control:cDNA sequencing results is available upon request.