Human CHGB Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13678-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CHGB cDNA Clone Datasheet

CHGB cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001819.2
cDNA Size:	2034
Gene Synonym:	SCG1;CHGB
Source:	Human

CHGB cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens chromogranin B DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 433 G/A, 727 A/G resulting in the amino acid Asp substitution by Asn, Thr substitution by Ala and 384 G/A not causing the amino acid variation.

CHGB cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CHGB cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CHGB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGCCAA CGCTGCTTCT CAGCCTCCTG GGAGCCGTGG GGCTGGCGGC TGTCAATTCC
61       ATGCCAGTGG ATAACAGGAA CCACAATGAA GGAATGGTGA CTCGCTGCAT CATTGAGGTC
121      CTCTCAAATG CCTTGTCGAA GTCCAGCGCT CCACCCATCA CCCCTGAGTG CCGCCAAGTC
181      CTGAAGACGA GTAGAAAAGA CGTCAAAGAC AAAGAGACAA CTGAAAATGA AAACACAAAG
241      TTTGAAGTAA GATTGTTAAG AGACCCAGCT GATGCCTCGG AAGCCCACGA GTCCTCCAGC
301      AGGGGAGAGG CAGGAGCCCC AGGGGAGGAG GACATCCAAG GCCCAACAAA GGCAGACACA
361      GAGAAATGGG CAGAGGGAGG CGGACACAGC CGAGAGCGAG CGGATGAGCC CCAGTGGAGC
421      CTCTATCCCT CCAACAGCCA AGTCTCTGAA GAAGTGAAGA CACGCCATTC TGAGAAGAGC
481      CAGAGAGAGG ATGAGGAGGA GGAGGAGGGA GAGAACTATC AAAAAGGGGA GCGAGGGGAA
541      GATAGCAGTG AAGAGAAACA CCTTGAAGAG CCAGGAGAGA CACAAAACGC TTTTCTCAAT
601      GAAAGAAAGC AGGCTTCAGC TATAAAAAAA GAGGAGTTAG TGGCCAGATC GGAAACACAT
661      GCTGCCGGGC ATTCTCAGGA GAAGACACAT AGCCGAGAGA AGAGTAGCCA GGAGAGTGGA
721      GAGGAGGCAG GGAGCCAGGA GAATCACCCC CAGGAGTCTA AAGGCCAACC CCGAAGCCAG
781      GAAGAATCTG AGGAAGGTGA GGAAGATGCC ACCTCTGAGG TGGACAAACG ACGCACGAGG
841      CCCAGACACC ACCACGGGAG GAGCAGGCCC GACAGGTCCT CTCAAGGAGG GAGTCTTCCC
901      TCTGAGGAAA AGGGACACCC CCAGGAGGAA TCTGAGGAGT CAAACGTCAG CATGGCCAGT
961      TTAGGGGAAA AGAGGGACCA CCATTCAACC CACTACAGGG CTTCAGAGGA AGAACCTGAA
1021     TATGGAGAAG AAATAAAGGG TTATCCAGGC GTCCAGGCCC CTGAGGACCT GGAGTGGGAG
1081     CGCTATAGGG GCAGAGGAAG TGAAGAATAC AGGGCTCCAA GACCTCAGAG TGAGGAGAGT
1141     TGGGATGAGG AGGACAAGAG AAACTACCCC AGCTTAGAGC TTGATAAGAT GGCACATGGA
1201     TATGGTGAAG AAAGTGAGGA AGAGAGGGGC CTTGAGCCGG GAAAGGGACG CCATCACAGA
1261     GGCAGGGGAG GGGAGCCACG TGCCTATTTC ATGTCTGACA CCAGAGAAGA GAAAAGGTTC
1321     TTGGGTGAAG GACACCACCG TGTCCAAGAA AACCAGATGG ACAAGGCAAG GAGGCATCCA
1381     CAAGGTGCGT GGAAAGAGCT GGACAGAAAT TATCTCAACT ACGGTGAGGA AGGAGCCCCA
1441     GGGAAGTGGC AGCAGCAGGG AGACCTGCAG GACACTAAAG AAAACAGGGA GGAAGCTAGG
1501     TTTCAAGATA AACAATATAG CTCCCATCAC ACAGCTGAAA AGAGGAAGAG ATTAGGGGAA
1561     CTGTTCAACC CATACTACGA CCCTCTCCAG TGGAAGAGCA GCCATTTTGA AAGAAGAGAC
1621     AACATGAATG ACAATTTTCT CGAGGGTGAG GAGGAAAATG AGCTGACCTT GAACGAGAAG
1681     AATTTCTTCC CAGAATACAA CTATGACTGG TGGGAGAAAA AGCCCTTCTC TGAGGATGTG
1741     AACTGGGGGT ATGAGAAGAG AAACCTCGCC AGGGTCCCCA AGCTGGACCT GAAAAGGCAA
1801     TATGACAGGG TGGCCCAACT GGACCAGCTC CTTCACTACA GGAAGAAGTC AGCTGAGTTT
1861     CCAGACTTCT ATGATTCTGA GGAGCCGGTG AGCACCCACC AGGAGGCAGA AAATGAAAAG
1921     GACAGGGCTG ACCAGACAGT CCTGACAGAG GACGAGAAAA AAGAACTCGA AAACTTGGCT
1981     GCAATGGATT TGGAACTACA GAAGATAGCT GAGAAATTCA GCCAAAGGGG CTGA

Quality Control:cDNA sequencing results is available upon request.