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Human CHAT Gene cDNA Clone (full-length ORF Clone)
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Human CHAT Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG15748-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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CHAT cDNA Clone Datasheet

 

CHAT cDNA Clone Product Information

Gene Bank Ref.ID: BC130615

cDNA Size:

1893

Gene Synonym:

CMS1A, CMS1A2, CHOACTASE

Source: Human

CHAT cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens choline O-acetyltransferase DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

CHAT cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CHAT cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CHAT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG
61       CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
121      TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
181      CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
241      CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
301      ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
361      ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
421      GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
481      TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
541      CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
601      ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
661      ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
721      AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
781      GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
841      AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
901      AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
961      GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
1021     AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
1081     GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
1141     GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
1201     TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
1261     CTCCAGCTGG CCTTCTACAG GCTCCACCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
1321     ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
1381     GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
1441     CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
1501     GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
1561     CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
1621     CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
1681     GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
1741     TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
1801     AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
1861     GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

 

Quality Control:cDNA sequencing results is available upon request.


 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"