Human CGREF1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13360-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CGREF1 cDNA Clone Datasheet

CGREF1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC034764
cDNA Size:	906
Gene Synonym:	CGR11; CGREF1
Source:	Human

CGREF1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens cell growth regulator with EF-hand domain 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CGREF1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CGREF1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CGREF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTACCTT TGACGATGAC AGTGTTAATC CTGCTGCTGC TCCCCACGGG TCAGGCTGCC
61       CCAAAGGATG GAGTCACAAG GCCAGACTCT GAAGTGCAGC ATCAGCTCCT GCCCAACCCC
121      TTCCAGCCAG GCCAGGAGCA GCTCGGACTT CTGCAGAGCT ACCTAAAGGG ACTAGGAAGG
181      ACAGAAGTGC AACTGGAGCA TCTGAGCCGG GAGCAGGTTC TCCTCTACCT CTTTGCCCTC
241      CATGACTATG ACCAGAGTGG ACAGCTGGAT GGCCTGGAGC TGCTGTCCAT GTTGACAGCT
301      GCTCTGGCCC CTGGAGCTGC CAACTCTCCT ACCACCAACC CGGTGATATT GATAGTGGAC
361      AAAGTGCTCG AGACGCAGGA CCTGAATGGG GATGGGCTCA TGACCCCTGC TGAGCTCATC
421      AACTTCCCGG GAGTAGCCCT CAGGCACGTG GAGCCCGGAG AGCCCCTTGC TCCATCTCCT
481      CAGGAGCCAC AAGCTGTTGG AAGGCAGTCC CTATTAGCTA AAAGCCCATT AAGACAAGAA
541      ACACAGGAAG CCCCTGGTCC CAGAGAAGAA GCAAAGGGCC AGGTAGAGGC CAGAAGGGAG
601      TCTTTGGATC CTGTCCAGGA GCCTGGGGGC CAGGCAGAGG CTGATGGAGA TGTTCCAGGG
661      CCCAGAGGGG AAGCTGAGGG CCAGGCAGAG GCTAAAGGAG ATGCCCCTGG GCCCAGAGGG
721      GAAGCTGGGG GCCAGGCAGA GGCTGAAGGA GATGCCCCCG GGCCCAGAGG GGAAGCTGGG
781      GGCCAGGCAG AGGCCAGGGA GAATGGAGAG GAGGCCAAGG AACTTCCAGG GGAAACACTG
841      GAGTCTAAGA ACACCCAAAA TGACTTTGAG GTGCACATTG TTCAAGTGGA GAATGATGAG
901      ATCTAG

Quality Control:cDNA sequencing results is available upon request.