CFL2 / cofilin 2 / ADF Protein (His Tag)

Cofilin 2 (muscle) Protein Datasheet

CFL2 / cofilin 2 / ADF Protein Product Information

• Synonym: NEM7
• Protein Construction: A DNA sequence encoding the human CFL2 (Q9Y281-1) (Ala 2-Leu 166) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E. Coli

CFL2 / cofilin 2 / ADF Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CFL2 consisting of 180 amino acids and has a calculated molecular mass of 20.4 kDa. The apparent molecular mass of the protein is approximately 21KDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CFL2 / cofilin 2 / ADF protein

CFL2 / cofilin 2 / ADF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CFL2 / cofilin 2 / ADF Protein Description

Cofilin 2 (CFL2) or ADF is a member of the cofilin family. It shows significant homology to a conventional Dictyostelium cofilin, cofilin 1, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) are important regulators of striated myocyte function. Several residues that are conserved among the cofilin family are missing from cofilin 2. Purified cofilin 2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin 2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells.

References

1. Aizawa H. et al., 2001, Genes Cells. 6 (10): 913-21.
2. Papalouka V. et al., 2009,Papalouka V Mol Cell Biol. 29 (22): 6046-58.