CFHR1 Protein (His Tag)

Complement factor H-related 1 Protein Datasheet

CFHR1 Protein Product Information

• Synonym: CFHR1, CFHL, CFHL1, CFHL1P, CFHR1P, FHR1, HFL1, HFL2
• Protein Construction: A DNA sequence encoding the human CFHR1 (Q03591) (Met1-Arg330) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

CFHR1 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 19
• Molecular Mass: The recombinant human CFHR1 consists of 323 amino acids and predicts a molecular mass of 37.2 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CFHR1 protein

CFHR1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CFHR1 Protein Description

CFHR1 is a member of the complement factor H protein family. It is a secreted protein which binds to Pseudomonas aeruginosa elongation factor Tuf together with plasminogen, which is proteolytically activated. It is proposed that Tuf acts as a virulence factor by acquiring host proteins to the pathogen surface, controlling complement, and facilitating tissue invasion. The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1(FHL-1) and five factor H-related proteins (CFHR-1 to -5). CFHR1 might be involved in complement regulation. It can associate with lipoproteins and may play a role in lipid metabolism.

References

1. Martínez-Barrica. et al., 2012, Invest Ophthalmol Vis Sci. 53 (3): 1087-94.
2. Leban N. et al., 2012, Int J Immunogenet. 39 (2): 110-3.
3. Kubista KE. et al., 2011, Mol Vis. 17: 2080-92.