>Rabbit PAb Antibody
>CES3 / Carboxylesterase 3 Antibody (Antigen Affinity Purified)
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CES3 / Carboxylesterase 3 Antibody ( Antigen Affinity Purified ) Datasheet
|Order or Inquire for CES3 / Carboxylesterase 3 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.5 ng/lane in WB|
|Detection limit is 0.00245 ng/well in ELISA|
CES3 / Carboxylesterase 3 Antibody Product Information
Recombinant Human CES3 / Carboxylesterase 3 protein (Catalog#11129-H08H)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Human CES3 / Carboxylesterase 3 (rh CES3 / Carboxylesterase 3; Catalog#11129-H08H; NP_079198.2; Met 1-Glu 561). CES3 / Carboxylesterase 3 specific IgG was purified by Human CES3 / Carboxylesterase 3 affinity chromatography.
CES3 / Carboxylesterase 3 Antibody Usage Guide
Human CES3 / Carboxylesterase 3
|Western blot :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CES3 in WB. Using a DAB detection system, the detection limit for Human CES3 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.|
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CES3. The detection limit for Human CES3 is approximately 0.00245 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
CES3 / Carboxylesterase 3 Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|CES3/Carboxylesterase 3||Human||CES3/Carboxylesterase 3 Protein, Recombinant||11129-H08H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human CES3/Carboxylesterase 3||WB, ELISA||CES3 / Carboxylesterase 3 Antibody||11129-MM07|
|Human CES3/Carboxylesterase 3||WB, ELISA||CES3 / Carboxylesterase 3 Antibody||11129-RP01|
|Human CES3/Carboxylesterase 3||WB, ELISA||CES3 / Carboxylesterase 3 Antibody (Antigen Affinity Purified)||11129-RP02|
|Human CES3/Carboxylesterase 3||WB, ELISA||CES3 / Carboxylesterase 3 Antibody||11129-R372|
CES3 / Carboxylesterase 3 Antibody Background
Carboxylesterases hydrolyze esters of short-chain fatty acids and have roles in animals ranging from signal transduction to xenobiotic detoxification. In enzymology, a carboxylesterase is an enzyme that catalyzes the chemical reaction: a carboxylic ester + H2O = an alcohol + a carboxylate. Most enzymes from this group belong to the superfamily of hydrolases with alpha/beta protein fold (so called Alpha/beta hydrolase fold), specifically those acting on carboxylic ester bonds. The carboxylesterase family of evolutionarily related proteins (those with clear sequence homology to each other) includes a number of proteins with different substrate specificities, such as acetylcholinesterases. Carboxylesterase 3, also known as Liver carboxylesterase 31 homolog and CES3, is a endoplasmic reticulum lumen which belongs to thetype-B carboxylesterase/lipase family. CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES3 shows low catalytic efficiency for hydrolysis of CPT-11, a prodrug for camptothecin used in cancer therapeutics. CES3 is expressed in liver, colon and small intestine.
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