Human CERK Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12264-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CERK cDNA Clone Datasheet

CERK cDNA Clone Product Information

Gene Bank Ref.ID:	BC004278
cDNA Size:	606
Gene Synonym:	LK4; hCERK; dA59H18.2; dA59H18.3; CERK
Source:	Human

CERK cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens ceramide kinase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CERK cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CERK cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CERK cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAACGTG GCCCTGCTTC AGGTGATCCG CGGGAGGGGC CTCCACGCCA GCGCCGGGAA
61       GGCTGCTGGG GCCTCCACAC CTGCCTCATC ACGGCGGCGA GGCTACGACA ATCCGGCTGG
121      GAGCATGACC TTGGCGTCTG TTCTGGGAGC ACGGATGATA AGCTCTGGAA GCTGGCAGTG
181      TGTAAAGCAC TGGCAAGTTT GTTACTGTTA AAATGTCAAA TACCAATGCT TTATATCGAC
241      GCGAAGTGCT TAACACAGCC GGGCTTGGGG GCAGTCAGGA GGAAGCTGGC CATCCGTGGA
301      GGAGGGGCCG GTCCTGGACT CCCGCAGGAC TCCTCTGAGG CAGGGCCTGA AGTCTGTACA
361      CGTGGTCCAG ATTTGTCCTT GTCTTTTCTT CACACTGAGT TCTCTATATT TATTGAACAT
421      CTTGTCCTTT TAAGCCAGAG TAGTGTAAAC TGCGTCTCGG ATGTCTGTCT TTTGCCTCGA
481      AGCCACGATG GATCGCTGGT TTCCTCTGCA GCGCGAGGGC TCCGGCGACC AGAGGATTCT
541      TCCCGGAAGG CATTCCTGCC GCGCTCCCCG GGGCACCCCT CAATTGTGTA CTACGTCCTT
601      GTTTAG

Quality Control:cDNA sequencing results is available upon request.