Anti-CD66b Antibody

Cat: 11729-MM04
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Anti-CD66b Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD66b Antibody
Validated applications
ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human CD66b
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Immunogen
Recombinant Human CEACAM8 / CD66b protein (Catalog#11729-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CEACAM8 / CD66b (rh CEACAM8 / CD66b; Catalog#11729-H08H; NP_001807.2; Met 1-Ser 319). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #04
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD66b Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD66b Antibody (Mouse Monoclonal antibody) Images
Human CEACAM8/CD66b/CD67 Flow Cytometry (FC) 2546
Profile of peripheral blood granulocytes analyzed by flow cytometry.
Anti-CD66b Antibody Alternative Names
Anti-CD66b Antibody;Anti-CD67 Antibody;Anti-CGM6 Antibody;Anti-NCA-95 Antibody
CD66b Background Information

CEACAM8, also known as CD66b or NCA-95, is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic antigen family. There are four members in this family: CD66a, CD66b, CD66c, and CD66d. Members of CEACAM family are widely expressed especially on human neutrophils, and, depending on the tissue, capable of regulating diverse functions including tumor promotion, tumor suppression, angiogenesis, and neutrophil activation. Abnormal overexpression and downregulation of some CEACAMs have been described in tumor cells. Monoclonal antibodies grouped in the CD66 cluster recognize CEACAM members. Ectopic CD66 expression is commonly detected in B-cell lineage acute lymphoblastic leukemia (ALL). CEACAM8(CD66b) is also an activation marker for human granulocytes. However, its biological functions are largely unknown in eosinophils. It has been reported that CD66b is highly expressed on the surface of human peripheral blood eosinophils isolated from healthy individuals. Engagement of CD66b by mAb or a natural ligand, galectin-3, activated a Src kinase family molecule, hemopoietic cell kinase (Hck), and induced cellular adhesion, superoxide production, and degranulation of eosinophils. CD66b molecules were localized in lipid rafts, and disruption of lipid rafts or removal of the GPI anchor inhibited the adhesion and activation of eosinophils. Importantly, CD66b was constitutively and physically associated with a beta2 integrin, CD11b, and cross-linking of CD66b induced a striking clustering of CD11b molecules. Thus, CD66b molecules are involved in regulating adhesion and activation of eosinophils, possibly through their localization in lipid rafts and interaction with other cell surface molecules, such as CD11b. Binding of exogenous or endogenous carbohydrate ligands(s) to CD66b may be important in the release of proinflammatory mediators by human eosinophils.

Full Name
carcinoembryonic antigen-related cell adhesion molecule 8
References
  • Yoon J, et al. (2007) CD66b regulates adhesion and activation of human eosinophils. J Immunol. 179 (12): 8454-62.
  • Skubitz KM, et al. (1996) CD6a, CD66b, CD66c, and CD66d each independently stimulate neutrophils. J Leukoc Biol. 60 (1): 106-17.
  • Skubitz KM, et al. (2008) Inte rdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells. J Transl Med. 6: 78.
  • Lasa A, et al. (2008) High expression of CEACAM6 and CEACAM8 mRNA in acute lymphoblastic leukemias. Ann Hematol. 87 (3): 205-11.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD66b Antibody Rabbit Monoclonal ELISA 11729-R013
    Anti-CD66b Antibody Rabbit Polyclonal ELISA 11729-RP01
    Anti-CD66b Antibody Rabbit Polyclonal ELISA 11729-RP02
    Anti-CD66b Antibody (APC) Mouse Monoclonal FCM 11729-MM04-A
    Anti-CD66b Antibody (FITC) Mouse Monoclonal FCM 11729-MM04-F
    Anti-CD66b Antibody (PE) Mouse Monoclonal FCM 11729-MM04-P
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