Anti-CEACAM6 Antibody

Cat: 10823-R408
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Anti-CEACAM6 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CEACAM6 Antibody
Validated applications
WB,ELISA,FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human CEACAM6
No cross-reactivity in ELISA with Human CEACAM1 / CD66a Human CEACAM5 / CD66e
Immunogen
Recombinant Human CD66c protein (Catalog#10823-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CEACAM6 (rhCEACAM6; Catalog#10823-H08H; NP_002474.3; Met 1-Gly 320).
Source
Monoclonal Rabbit IgG Clone #408
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CEACAM6 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
ICC-IF 1:100-1:500  
FCM 1:100-1:500  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CEACAM6 Antibody (Rabbit Monoclonal antibody) Images
Human CEACAM6/CD66c Flow Cytometry (FC) 5214
Flow cytometric analysis of Human CEACAM6 expression on human peripheral blood granulocytes. Cells were stained with purified anti-Human CEACAM6, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.
Human CEACAM6/CD66c Western blot (WB) 14770

Anti-CEACAM6 rabbit monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:37 kDa

Observed band size:52 kDa

Human CEACAM6/CD66c Immunofluorescence(IF) 14799
Immunofluorescence staining of Human CEACAM6 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CEACAM6 monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Anti-CEACAM6 Antibody Alternative Names
Anti-CD66c Antibody;Anti-CEA Antibody;Anti-CEAL Antibody;Anti-NCA Antibody
CEACAM6 Background Information

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), also known as nonspecific crossreacting antigen (NCA) and CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily. It s a glycosylphosphatidylinositol-linked immunoglobulin superfamily member that is overexpressed in a variety of human cancers, including colon, breast and lung and is associated with tumourigenesis, tumour cell adhesion, invasion and metastasis. CEACAM6 is a unique mediator of migration and invasion of drug resistant oestrogen-deprived breast cancer cells, and this protein could be an important biomarker of metastasis. CEACAM6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.

Full Name
carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen)
References
  • Duxbury MS, et al. (2004) Overexpression of CEACAM6 promotes insulin-like growth factor I-induced pancreatic adenocarcinoma cellular invasiveness. Oncogene. 23(34): 5834-42.
  • Lewis-Wambi JS, et al. (2008) Overexpression of CEACAM6 promotes migration and invasion of oestrogen-deprived breast cancer cells. Eur J Cancer. 44(12): 1770-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CEACAM6 Antibody Rabbit Polyclonal ELISA 10823-RP04
    Anti-CEACAM6 Antibody Mouse Monoclonal FCM 10823-MM12
    Anti-CEACAM6 Antibody (PE) Mouse Monoclonal FCM 10823-MM12-P
    Anti-CEACAM6 Antibody Mouse Monoclonal ELISA 10823-MM06
    Anti-CEACAM6 Antibody (PerCP) Rabbit Monoclonal FCM 10823-R408-C
    Anti-CEACAM6 Antibody Mouse Monoclonal ELISA,FCM 10823-MM05
    Anti-CEACAM6 Antibody Rabbit Monoclonal ELISA 10823-RM01
    Anti-CEACAM6 Antibody Rabbit Monoclonal ELISA(Det) 10823-R341
    Anti-CEACAM6 Antibody (PE) Rabbit Monoclonal FCM 10823-R408-P
    Anti-CEACAM6 Antibody (APC) Mouse Monoclonal FCM 10823-MM12-A
    Anti-CEACAM6 Antibody Rabbit Polyclonal ELISA 10823-RP01
    Anti-CEACAM6 Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 10823-RM03
    Anti-CEACAM6 Antibody (APC) Rabbit Monoclonal FCM 10823-R408-A
    Anti-CEACAM6 Antibody (PE) Mouse Monoclonal FCM 10823-MM05-P
    Anti-CEACAM6 Antibody (FITC) Rabbit Monoclonal FCM 10823-R408-F
    Anti-CEACAM6 Antibody Rabbit Polyclonal ELISA,IHC-P 10823-T24
    Anti-CEACAM6 Antibody (FITC) Rabbit Monoclonal FCM 10823-RM03-F
    Anti-CEACAM6 Antibody Rabbit Monoclonal ELISA 10823-RM02
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