CEACAM5 / CD66e Antibody

CEACAM5 / CD66e Antibody Datasheet

CEACAM5 / CD66e Antibody Product Information

• Immunogen: Recombinant Human CD66e protein (Catalog#11077-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 1B8E7
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CEACAM5 / CD66e (rh CEACAM5 / CD66e; Catalog#11077-H08H; NP_004354.2; Met 1-Ala 685). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CEACAM5 / CD66e Antibody Usage Guide

• Specificity: Human CEACAM5 / CD66e
• No cross-reactivity in ELISA with

  Human CEACAM1

  Human CEACAM6

  Human CEACAM3

  Human CEACAM8

  Human cell lysate (293 cell line)
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CEACAM5. The detection limit for Human CEACAM5 is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CEACAM5 / CD66e Antibody Background

CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domain and a GPI anchor, and exists as a homodimer. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli, and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.

References

1. Schrewe H. et al., 1990, Mol. Cell. Biol. 10: 2738-48.
2. Korotkova N. et al., 2008, Mol. Microbiol. 67: 420-34.
3. Taheri M. et al., 2000, J. Biol. Chem. 275: 26935-43.
4. Boehm M.K. et al., 2000, FEBS Lett. 475: 11-6.