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CEACAM1 / CD66a Antibody, Mouse MAb

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Human CEACAM1 Antibody Product Information
Immunogen:Recombinant Human CD66a protein (Catalog#10822-H08H)
Clone ID:1F11A6F11
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CEACAM1 / CD66a (rh CEACAM1; Catalog#10822-H08H; NP_001020083.1; Met 1-Gly 428). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CEACAM1 Antibody Usage Guide
Specificity:Human CEACAM1 / CD66a
No cross-reactivity with Human CEACAM5, Human CEACAM6 and Human cell lysate (293 cell line) in WB and ELISA
Application:ELISA, IHC-P

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CEACAM1 / CD66a. The detection limit for Human CEACAM1 / CD66a is approximately 0.16 ng/well.

This antibody can be used at 1-10 μg/mL with the appropriate secondary reagents to detect human CEACAM1 / CD66a by immunohistochemistry (paraffin-embedded sections).

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CEACAM1 Antibody IHC Application Image
CEACAM1 / CD66a Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Immunohistochemical staining of formalin fixed, paraffin-embedded human colon showing epithelial cells staining (1 μg/mL).
Other CEACAM1 Antibody Products
CEACAM‑1/CD66a Background

The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

Human CEACAM‑1/CD66a References
  • Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.
  • Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.
  • Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
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