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CEACAM1 / CD66a Antibody, Mouse MAb

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Human CEACAM1 Antibody Product Information
Immunogen:Recombinant Human CD66a protein (Catalog#10822-H08H)
Clone ID:8B6E2F4
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CEACAM1 / CD66a (rh CEACAM1; Catalog#10822-H08H; NP_001020083.1; Met 1-Gly 428). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CEACAM1 Antibody Usage Guide
Specificity:Human CEACAM1 / CD66a
No cross-reactivity in WB and ELISA with
Human cell lysate ( 293 cell line )
Application:ELISA, IHC-P, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CEACAM1. The detection limit for Human CEACAM1 is approximately 0.31 ng/well.

IHC-P: 10-20 µg/mL

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CEACAM1 Antibody IHC Application Image
CEACAM1 / CD66a Antibody, Mouse MAb, Immunohistochemistry
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Immunochemical Staining of human CEACAM1 in human colon carcinoma with Mouse Monoclonal Antibody (15 µg/m, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelial cells.
CEACAM1 / CD66a Antibody, Mouse MAb, Immunohistochemistry
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Immunochemical Staining of human CEACAM1 in human liver with Mouse Monoclonal Antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to bile canaliculus. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Human CEACAM1 Antibody FC Application Image
CEACAM1 / CD66a Antibody, Mouse MAb, Flow cytometric analysis
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Flow cytometric analysis of Human CEACAM1(CD66a) expression in HT-29 cells.
Other CEACAM1 Antibody Products
CEACAM‑1/CD66a Background

The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

Human CEACAM‑1/CD66a References
  • Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.
  • Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.
  • Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
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    Catalog: 10822-MM02-50
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    50 µg
    100 µg
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