Anti-CEACAM1 Antibody

Cat: 10822-MM02
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Anti-CEACAM1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CEACAM1 Antibody
Validated applications
ELISA,ELISA(Det),IHC-P,FCM
Species reactivity
Reacts with: Human
Not React with: Mouse, Rat
Specificity
Human CEACAM1
No cross-reactivity in ELISA with Human CEACAM5 Human CEACAM6
Immunogen
Recombinant Human CD66a protein (Catalog#10822-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CEACAM1 / CD66a (rh CEACAM1; Catalog#10822-H08H; NP_001020083.1; Met 1-Gly 428). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #8B6E2F4
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CEACAM1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
IHC-P 1:50-1:200  
FCM 1:25-1:100  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human CEACAM‑1 / CD66a in ELISA pair set (Catalog: # SEKA10822). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10822-R003).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CEACAM1 Antibody (Mouse Monoclonal antibody) Images
Human CEACAM‑1/CD66a Immunohistochemistry(IHC) 4443
Immunochemical Staining of human CEACAM1 in human liver with Mouse Monoclonal Antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to bile canaliculus. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Human CEACAM‑1/CD66a Immunohistochemistry(IHC) 4444
Immunochemical Staining of human CEACAM1 in human colon carcinoma with Mouse Monoclonal Antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelial cells.
Human CEACAM‑1/CD66a Flow Cytometry (FC) 5218
Flow cytometric analysis of Human CEACAM1(CD66a) expression in HT-29 cells.
Anti-CEACAM1 Antibody Alternative Names
Anti-BGP Antibody;Anti-BGP1 Antibody;Anti-BGPI Antibody;Anti-CD66a Antibody;Anti-CEACAM‑1 Antibody
CEACAM1 Background Information

The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

Immune Checkpoint
Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
References
  • Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.
  • Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.
  • Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CEACAM1 Antibody Rabbit Polyclonal ELISA,IHC-P 80224-T16
    Anti-CEACAM1 Antibody (PE) Mouse Monoclonal FCM 80224-MM03-P
    Anti-CEACAM1 Antibody (APC) Mouse Monoclonal FCM 80224-MM03-A
    Anti-CEACAM1 Antibody Mouse Monoclonal FCM 80224-MM03
    Anti-CEACAM1 Antibody Rabbit Polyclonal ELISA 80224-RP01
    Anti-CEACAM1 Antibody Mouse Monoclonal ELISA 80224-MM05
    Anti-CEACAM1 Antibody Rabbit Monoclonal IHC-P,IHC-Fr 50571-R030
    Anti-CEACAM1 Antibody (PerCP) Rabbit Monoclonal FCM 50571-R117-C
    Anti-CEACAM1 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 50571-R117
    Anti-CEACAM1 Antibody Rabbit Polyclonal ELISA,IHC-P,IHC-Fr 50571-RP02
    Anti-CEACAM1 Antibody Rabbit Polyclonal ELISA 50571-RP01
    Anti-CEACAM1 Antibody (FITC) Rabbit Monoclonal FCM 50571-R117-F
    Anti-CEACAM1 Antibody Rabbit Monoclonal ELISA 50571-R025
    Anti-CEACAM1 Antibody (APC) Rabbit Monoclonal FCM 50571-R117-A
    Anti-CEACAM1 Antibody (PE) Rabbit Monoclonal FCM 50571-R117-P
    Anti-CEACAM1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P 10822-RP04
    Anti-CEACAM1 Antibody Rabbit Monoclonal ELISA(Cap) 10822-R003
    Anti-CEACAM1 Antibody (APC) Mouse Monoclonal FCM 10822-MM02-A
    Anti-CEACAM1 Antibody (APC) Rabbit Monoclonal FCM 10822-R103-A
    Anti-CEACAM1 Antibody Rabbit Monoclonal ELISA 10822-R006
    Anti-CEACAM1 Antibody (PE) Mouse Monoclonal FCM 10822-MM02-P
    Anti-CEACAM1 Antibody Rabbit Polyclonal WB,ELISA 10822-RP01
    Anti-CEACAM1 Antibody (PE) Rabbit Monoclonal FCM 10822-R103-P
    Anti-CEACAM1 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 10822-R103
    Anti-CEACAM1 Antibody (FITC) Mouse Monoclonal FCM 10822-MM02-F
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