CDNF / ARMETL1 Antibody

CDNF / ARMETL1 Antibody Datasheet

CDNF / ARMETL1 Antibody Product Information

• Immunogen: Recombinant Mouse CDNF protein (Catalog#50569-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 030
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CDNF / ARMETL1 (rM CDNF / ARMETL1; Catalog#50569-M08H; NP_808315.1; Met 1-Leu 187).

CDNF / ARMETL1 Antibody Usage Guide

• Specificity: Mouse CDNF / ARMETL1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse ARMETL1 in WB. Using a DAB detection system, the detection limit for Mouse ARMETL1 is approximately 0.125 ng/lane under non-reducing conditions and 5 ng/lane under reducing conditions.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CDNF / ARMETL1 Antibody Background

Cerebral dopamine neurotrophic factor, also known as ARMET-like protein 1, Conserved dopamine neurotrophic factor, CDNF and ARMETL1, is a secreted protein which belongs to theARMET family. CDNF / ARMETL1 is widely expressed in neuronal and non-neuronal tissues. In the brain, highest levels of CDNF / ARMETL1 is expressed in the optic nerve and corpus callosum. Evolutionary conserved family of neurotrophic factors (NTFs) consisting of two members in the vertebrates, cerebral dopamine neurotrophic factor (CDNF) and mesencephalic astrocyte-derived neurotrophic factor (MANF). Invertebrates, including Drosophila and Caenorhabditis have a single protein homologous to vertebrate CDNF / MANF. Characteristic feature of these proteins is eight structurally conserved cysteine residues, which determine the protein fold. The crystal structure analysis revealed that CDNF and MANF consist of two domains; an amino-terminal saposin-like domain that may interact with lipids or membranes, and a presumably unfolded carboxy-terminal domain that may protect cells against endoplasmic reticulum stress. CDNF / ARMETL1 is a trophic factor for dopamine neurons. It prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, it restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra.

References

1. Lindholm,P. et al., 2007, Nature448 (7149): 73-7.
2. Apostolou A., et al. 2008, Exp. Cell Res. 314: 2454-67.
3. Parkash,V. et al., 2009, Protein Eng Des Sel22 (4): 233-41.
4. Lindholm,P. et al., 2010, Dev Neurobiol70 (5): 360-71.