Mouse CDK9/CDC2L4 Gene cDNA Clone / ORF Clone

CDK9/CDC2L4 cDNA Clone Datasheet

CDK9/CDC2L4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_130860.3
• cDNA Size: 1119
• Gene Synonym: PITALRE; Cdk9
• Source: Mouse

CDK9/CDC2L4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cyclin-dependent kinase 9 (CDC2-related kinase) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 361 T/C resulting in the amino acid Phe substitution by Leu.

CDK9/CDC2L4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDK9/CDC2L4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDK9/CDC2L4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAAGC AGTACGACTC GGTGGAATGC CCGTTCTGCG ATGAGGTCAC CAAGTACGAG
61       AAACTTGCCA AGATCGGCCA AGGCACATTC GGGGAAGTCT TTAAAGCCAA GCACCGTCAG
121      ACGGGCCAGA AGGTGGCTCT GAAGAAAGTG TTGATGGAGA ATGAGAAGGA GGGGTTCCCC
181      ATCACAGCCT TGAGGGAAAT CAAGATTCTA CAGCTCCTAA AACATGAGAA TGTGGTGAAC
241      CTAATTGAGA TTTGTCGGAC CAAAGCCTCA CCGTATAACC GCTGCAAGGG CAGCATCTAT
301      CTGGTGTTTG ACTTCTGTGA GCATGACCTT GCTGGGCTGC TGAGCAACGT CTTAGTCAAG
361      CTCACGTTGT CTGAGATCAA GAGAGTGATG CAGATGCTGC TGAACGGCCT CTACTACATC
421      CACAGGAACA AGATCCTGCA CAGGGACATG AAGGCTGCGA ATGTGCTCAT TACCCGGGAT
481      GGGGTCCTGA AGCTGGCAGA TTTTGGGCTG GCCCGTGCCT TCAGCCTAGC TAAGAATAGC
541      CAGCCCAACC GATACACCAA CCGTGTGGTG ACATTGTGGT ACCGGCCTCC GGAGCTGCTG
601      CTCGGAGAGC GGGACTACGG CCCGCCCATT GACCTTTGGG GTGCTGGGTG CATCATGGCA
661      GAGATGTGGA CTCGTAGCCC TATCATGCAG GGTAACACAG AGCAGCACCA GCTCGCCCTC
721      ATCAGCCAGC TCTGTGGCTC CATCACCCCA GAGGTGTGGC CAAACGTAGA CAAGTATGAG
781      CTGTTTGAAA AGCTGGAACT TGTGAAGGGC CAGAAGCGGA AGGTGAAGGA CCGTCTGAAG
841      GCCTACGTGC GCGACCCCTA TGCTCTGGAC CTCATTGACA AGCTGCTGGT GCTGGACCCC
901      GCGCAGCGGA TTGACAGTGA CGATGCCCTC AACCACGACT TCTTCTGGTC GGACCCCATG
961      CCCTCGGACC TCAAGGGCAT GCTGTCTACT CACTTGACGT CTATGTTTGA GTACCTGGCA
1021     CCGCCACGGC GGAAGGGCAG CCAGATCACC CAGCAGTCCA CCAACCAGAG CAGAAATCCC
1081     GCCACCACCA ACCAGACGGA ATTTGAACGT GTCTTCTGA

 

Quality Control:cDNA sequencing results is available upon request.