Mouse CDK5R1 Gene cDNA Clone / ORF Clone

CDK5R1 cDNA Clone Datasheet

CDK5R1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009871.2
• cDNA Size: 924
• Gene Synonym: p25; p35; Cdk5r; D11Bwg0379e; Cdk5r1
• Source: Mouse

CDK5R1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cyclin-dependent kinase 5, regulatory subunit 1 (p35) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 145 G/A resulting in the amino acid Val substitution by Met.

CDK5R1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDK5R1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDK5R1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCACGG TGCTGTCCCT ATCCCCCAGC TATCGGAAGG CCACACTGTT TGAGGATGGC
61       GCGGCCACCG TGGGCCACTA CACGGCCGTG CAGAACAGCA AGAACGCCAA GGACAAGAAC
121      CTGAAGCGGC ACTCCATCAT CTCGATGCTG CCTTGGAAGA GGATCGTGGC GGTGTCAGCG
181      AAGAAGAAGA ACTCCAAGAA GGCGCAGCCC AACAGCAGCT ACCAGAGCAA CATCGCGCAT
241      CTCAACAATG AGAACCTGAA GAAGTCGCTG TCCTGTGCCA ACCTGTCCAC ATTCGCCCAG
301      CCCCCGCCGG CTCAGCCGCC CGCACCCCCA GCCAGCCAGC TTTCTGGCTC CCAGACTGGG
361      GTCTCCTCTT CTGTCAAGAA GGCCCCGCAC CCTGCCATCA CCTCTGCAGG GACACCCAAA
421      CGGGTCATCG TCCAGGCGTC CACTAGTGAG CTGCTGCGCT GCCTGGGTGA GTTTCTCTGC
481      CGCCGGTGCT ACCGCCTGAA GCACTTGTCC CCAACGGACC CCGTGCTCTG GCTGCGCAGC
541      GTGGACCGCT CCCTGCTTCT GCAGGGCTGG CAGGACCAGG GTTTCATCAC ACCGGCCAAT
601      GTGGTCTTCC TCTACATGCT CTGCAGGGAT GTCATCTCCT CTGAGGTGGG CTCCGACCAT
661      GAGCTCCAGG CTGTCCTGCT GACCTGTCTG TACCTCTCCT ATTCCTACAT GGGCAATGAG
721      ATCTCCTACC CGCTCAAGCC CTTCCTGGTA GAGAGCTGTA AGGAAGCCTT TTGGGACCGT
781      TGCCTCTCAG TTATCAACCT CATGAGCTCC AAGATGCTGC AGATCAATGC CGACCCACAC
841      TATTTCACAC AAGTGTTCTC TGACTTGAAG AATGAGAGCG GTCAGGAGGA CAAGAAGCGA
901      CTCCTCCTGG GGCTGGATCG GTGA

 

Quality Control:cDNA sequencing results is available upon request.