Mouse CDK4 Gene cDNA Clone / ORF Clone

CDK4 cDNA Clone Datasheet

CDK4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009870.3
• cDNA Size: 912
• Gene Synonym: Crk3; Cdk4
• Source: Mouse

CDK4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cyclin-dependent kinase 4 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CDK4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDK4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDK4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCCA CTCGATATGA ACCCGTGGCT GAAATTGGTG TCGGTGCCTA TGGGACGGTG
61       TACAAAGCCC GAGATCCCCA CAGTGGCCAC TTTGTGGCCC TCAAGAGTGT GAGAGTTCCT
121      AATGGAGGAG CAGCTGGAGG GGGCCTTCCC GTCAGCACAG TTCGTGAGGT GGCCTTGTTA
181      AGGAGGCTGG AGGCCTTTGA ACATCCCAAT GTTGTACGGC TGATGGATGT CTGTGCTACT
241      TCCCGAACTG ATCGGGACAT CAAGGTCACC CTAGTGTTTG AGCATATAGA CCAGGACCTG
301      AGGACATACC TGGACAAAGC ACCTCCACCG GGCCTGCCGG TTGAGACCAT TAAGGATCTA
361      ATGCGTCAGT TTCTAAGCGG CCTGGATTTT CTTCATGCAA ACTGCATTGT TCACCGGGAC
421      CTGAAGCCAG AGAACATTCT AGTGACAAGT AATGGGACCG TCAAGCTGGC TGACTTTGGC
481      CTAGCTAGAA TCTACAGCTA CCAGATGGCC CTCACGCCTG TGGTGGTTAC GCTCTGGTAC
541      CGAGCTCCTG AAGTTCTTCT GCAGTCTACA TACGCAACAC CCGTGGACAT GTGGAGCGTT
601      GGCTGTATCT TTGCAGAGAT GTTCCGTCGG AAGCCTCTCT TCTGTGGAAA CTCTGAAGCC
661      GACCAGTTGG GGAAAATCTT TGATCTCATT GGATTGCCTC CAGAAGACGA CTGGCCTCGA
721      GAGGTATCTC TACCTCGAGG AGCCTTTGCC CCCAGAGGGC CTCGGCCAGT GCAGTCAGTG
781      GTGCCAGAGA TGGAGGAGTC TGGAGCGCAG CTGCTACTGG AAATGCTGAC CTTTAACCCA
841      CATAAGCGAA TCTCTGCCTT CCGAGCCCTG CAGCACTCCT ACCTGCACAA GGAGGAAAGC
901      GACGCAGAGT GA

 

Quality Control:cDNA sequencing results is available upon request.