Rat CDH2 Gene cDNA Clone / ORF Clone

CDH2 cDNA Clone Datasheet

CDH2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031333.1
• cDNA Size: 2721
• Gene Synonym: N-cadherin; Cdh2
• Source: Rat

CDH2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus cadherin 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 457-458 CG/GC, 1974 G/T resulting in the amino acid Arg substitution by Ala, Lys substitution by Asn and 1941 C/T，2082 G/C，2430 C/T，2457 A/G not causing the amino acid variation.

CDH2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGCCGGA TAGCGGGAGC GCCGCGGACC CTGCTGCCGC TTCTGGCGGC CTTGCTTCAG
61       GCATCTCTGG AGGCTTCTGG TGAACTTGCA TTATGCAAGA CTGGATTTCC TGAAGATGTT
121      TACAGCGCGG TCTTACCGAA GGACGTGCAC GAAGGCCAGC CTCTTCTCAA TGTGAAATTC
181      AGCAACTGCA ATAGAAAAAG GAAAGTTCAG TATGAAAGCA GTGAACCAGC AGATTTCAAG
241      GTGGATGAGG ACGGCACAGT GTACGCTGTG AGAAGCTTCC CTCTCTCTGC AGAGCAGGCA
301      AAGTTCCTGA TATATGCCCA AGACAAAGAA ACCCAGGAAA AGTGGCAGGT AGCTGTTAAC
361      CTGAGCCTGG AGCCCAGCCT GACTGAGGAG CCGATGAAGG AACCACATGA AATTGAAGAA
421      ATAGTATTCC CTAGACAACT CGCCAAGCAC AGCGGAGCTC TACAAAGGCA GAAGAGAGAC
481      TGGGTCATCC CTCCGATCAA CTTGCCAGAA AACTCCAGAG GACCCTTTCC GCAAGAGCTT
541      GTCAGAATCA GGTCTGATAG AGATAAGAAC CTATCTCTGA GATACAGTGT CACTGGGCCA
601      GGAGCTGACC AGCCTCCAAC GGGCATCTTC ATCATCAACC CCATCTCTGG ACAACTGTCA
661      GTCACAAAGC CTCTGGACAG AGAACTGATA GCCCGGTTTC ACCTGAGGGC ACATGCAGTT
721      GACATCAATG GCAATCAAGT GGAGAACCCC ATTGACATCG TCATCAATGT CATCGACATG
781      AATGATAACA GACCTGAGTT TCTGCACCAG GTTTGGAATG GGTCTGTTCC AGAGGGATCA
841      AAGCCTGGGA CGTATGTGAT GACTGTCACT GCCATTGATG CAGATGACCC AAATGCCCTG
901      AATGGGATGC TGAGGTACAG GATCCTCTCC CAGGCACCCA GCACCCCTTC TCCCAACATG
961      TTTACAATCA ACAACGAGAC CGGGGACATC ATCACTGTGG CAGCTGGTCT GGACCGAGAG
1021     AAAGTGCAAC AGTATACGTT AATAATTCAA GCCACTGACA TGGAAGGCAA TCCCACTTAC
1081     GGCCTTTCAA ACACAGCCAC AGCCGTCATC ACAGTGACAG ATGTCAATGA CAATCCTCCA
1141     GAGTTTACTG CCATGACCTT CTACGGAGAA GTCCCTGAGA ACAGAGTGGA TGTCATTGTA
1201     GCCAACCTGA CTGTCACCGA CAAGGACCAG CCCCACACAC CAGCCTGGAA TGCGGCATAC
1261     AGAATCAGTG GTGGAGACCC CACGGGAAGG TTTGCCATCC TGACAGACCC CAACAGCAAT
1321     GATGGGCTAG TCACAGTGGT AAAACCAATT GACTTTGAAA CGAATAGGAT GTTTGTCCTC
1381     ACTGTTGCTG CAGAAAACCA AGTGCCATTA GCTAAGGGGA TCCAGCACCC ACCTCAGTCG
1441     ACAGCCACGG TGTCTGTGAC AGTTATTGAT GTCAATGAAA ATCCTTATTT TGCCCCAAAT
1501     CCTAAAATCA TTCGCCAAGA GGAAGGCCTC CACGCAGGTA CCATGCTGAC CACTCTCACT
1561     GCTCAGGACC CCGATCGATA TATGCAACAG AATATCAGAT ACACAAAGTT ATCTGACCCT
1621     GCCAACTGGC TGAAAATAGA CCCTGTGAAC GGGCAGATCA CCACTATCGC TGTTCTGGAC
1681     AGAGAGTCGC CAAATGTCAA AAACAACATC TATAATGCTA CCTTCCTTGC TTCTGACAAT
1741     GGAATCCCTC CTATGAGTGG AACAGGAACG CTGCAAATCT ATTTACTTGA TATCAATGAC
1801     AACGCCCCTC AGGTGTTACC TCAAGAGGCC GAGACTTGTG AAACTCCAGA ACCCAATTCA
1861     ATTAACATCA CAGCGCTTGA TTATGACATA GACCCCAATG CAGGGCCCTT TGCATTTGAC
1921     CTTCCCTTAT CCCCAGTGAC TATTAAAAGA AACTGGACCA TCACCCGGCT TAATGGTGAT
1981     TTTGCTCAGC TCAATTTAAA GATCAAGTTT TTGGAAGCTG GCATCTATGA AGTTCCCATC
2041     GTTATCACAG ATTCAGGGAA TCCCCCCAAG TCCAATATTT CCATCCTGCG TGTGAAAGTT
2101     TGCCAATGTG ACTCCAATGG AGACTGCACC GACGTAGACA GGATCGTGGG TGCAGGGCTT
2161     GGCACGGGCG CCATCATCGC GATACTTCTG TGTATCATCA TCCTGCTGAT CCTTGTCCTC
2221     ATGTTCGTGG TATGGATGAA ACGCCGGGAT AAAGAGCGCC AGGCCAAGCA GCTTTTAATA
2281     GACCCAGAAG ATGATGTAAG AGATAACATA TTGAAATATG ATGAAGAAGG TGGAGGAGAG
2341     GAAGACCAGG ACTATGACTT GAGCCAGCTC CAGCAACCAG ATACCGTGGA GCCTGATGCC
2401     ATCAAACCTG TGGGAATCAG ACGGCTAGAT GAGAGGCCTA TCCACGCTGA GCCACAGTAT
2461     CCAGTCCGAT CCGCAGCCCC ACACCCTGGG GATATTGGGG ACTTCATTAA TGAGGGCCTT
2521     AAAGCTGCTG ACAATGACCC CACGGCGCCA CCATATGACT CCCTTTTAGT CTTCGACTAT
2581     GAGGGCAGCG GCTCCACGGC TGGCTCCTTG AGCTCTCTCA ACTCCTCCAG CAGCGGCGGG
2641     GACCAGGACT ATGACTACCT GAATGACTGG GGACCCCGCT TCAAGAAGCT GGCCGACATG
2701     TATGGTGGTG GTGATGACTG A

 

Quality Control:cDNA sequencing results is available upon request.