Cynomolgus monkey CDH18 Gene cDNA Clone / ORF Clone

CDH18 cDNA Clone Datasheet

CDH18 cDNA Clone Product Information

• cDNA Size: 2373
• Gene Synonym: CDH18
• Source: Cynomolgus monkey

CDH18 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) cadherin 18, type 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1029 T/C，1446 C/T，2004 G/A，2065 C/A and 2196 C/T not causing the amino acid variation.
  Please check the sequence information before order.

CDH18 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH18 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH18 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAAATTA CGAGCACATC TTGCATCTGT CCAGTCCTAG TGTGTCTCTG TTTTGTGCAG
61       AGGTGTTATG GAACCGCTCA CCACAGCTCC ATCAAGGTGA CGAGAAACCA AACCAAACAC
121      ATTGAAGGTG AGACCGAAGT CCACCATCGT CCCAAAAGGG GATGGGTATG GAATCAGTTC
181      TTTGTTTTAG AAGAACATAT GGGACCAGAT CCTCAGTATG TTGGAAAGCT TCACTCCAAT
241      TCTGACAAAG GTGATGGATC TGTCAAGTAC ATCCTTACTG GAGAGGGTGC TGGGACTATA
301      TTTATCATTG ACGATACCAC GGGTGATATC CACTCAACAA AAAGCCTAGA CAGAGAGCAG
361      AAGACCCACT ATGTGCTTCA CGCTCAAGCC ATTGACAGAC GTACAAACAA ACCTCTTGAA
421      CCTGAATCTG AGTTTATCAT CAAAGTGCAA GACATCAATG ACAATGCTCC AAAATTCACA
481      GATGGACCAT ACATTGTTAC TGTGCCTGAA ATGTCAGACA TGGGTACCTC TGTTCTACAG
541      GTGACAGCTA CTGATGCAGA TGACCCTACC TATGGAAACA GTGCTCGGGT GGTTTACAGC
601      ATTCTCCAGG GACAACCCTA CTTCTCCGTC GATCCTAAAA CAGGAGTTAT TAGAACGGCC
661      TTACATAACA TGGACAGAGA AGCCAGAGAA CATTACTCGG TGGTCATTCA AGCCAAAGAC
721      ATGGCTGGGC AAGTTGGAGG GCTTTCGGGG TCTACAACAG TCAACATCAC CTTAACCGAT
781      GTCAATGACA ACCCACCGCG CTTTCCTCAA AAACATTATC AGCTATATGT TCCTGAGTCA
841      GCTCAAGTTG GTTCAGCTGT TGGGAAAATC AAGGCAAATG ATGCCGATAC TGGCTCAAAT
901      GCTGACATGA CCTACTCCAT CATAAATGGT GATGGCATGG GGATATTCTC AATCTCCACT
961      GACAAAGAGA CCAGAGAAGG AATCCTTTCT TTAAAGAAGC CACTGAACTA TGAGAAAAAG
1021     AAGTCATACA CCCTCAACAT AGAAGGAGCA AATACACATC TTGATTTTCG CTTTTCTCAC
1081     CTGGGTCCTT TTAAAGATGC TACCATGCTG AAGATCATTG TTGGGGATGT AGATGAACCA
1141     CCACTCTTTT CCATGCCTTC CTACGTCATG GAAGTCTATG AAAATGCCAA GATCGGGACC
1201     GTCGTTGGTA CAGTTTTGGC ACAAGATCCT GACAGTGCTA ATAGCTTAGT AAGATACTTC
1261     ATCAACTACA ATGCTGAAGA TGACAGATTT TTCAACATTG ATGCCAATAC TGGAACCATT
1321     AAGACTACAA AGGTTCTTGA CAGAGAAGAA ACTCCATGGT ACAACATCAC AGTCACTGCT
1381     TCAGAAAATG ATAATCCTGA TTTGCTGAGC CATGTCACAG TGGGTATTAG AGTTCTGGAT
1441     GTCAATGACA ATCCACCCGA ACTTGCCAGG GAATATGATA TTGTTGTCTG TGAAAATTCT
1501     AAGCCTGGCC AGGTTATTCA CACCATCAGT GCCACTGATA AAGATGATTT TGCCAATGGA
1561     CCAAGGTTTA ACTTCTTTCT TGATGAACAC CTGCCTGTAA ATCCAAACTT CACTCTGAAG
1621     GACAATGAAG ATAACACAGC CAGCATTCTG ACAAGGCGGA GGAGATTTAG TCGAACTGTT
1681     CAGGATGTGT ATTATCTGCC CATTATGATC TCTGATGGTG GAATCCCCTC TCTCAGCAGC
1741     AGCAGCACCC TCACCATCAG GGTTTGTGCA TGCGAGAGAG ATGGGCGCGT GCGGACCTGC
1801     CATGCGGAAG CGTTTCTGTC CTCGGCTGGT TTGAGTACAG GAGCCTTAAT CGCTATTCTT
1861     CTCTGTGTTC TCATTCTCCT GGCAATTGTG GTAATTTTTA TCACCCTGAG GCGCAGCAAA
1921     AAAGAGCCCT TGATCATTTC AGAGGAGGAT GTACGGGAGA ACGTGGTCAC CTATGATGAT
1981     GAAGGAGGCG GAGAGGAAGA CACAGAGGCC TTTGACATCA CAGCCTTGAG GAATCCTTCT
2041     GCTGCTGAGG AGCTCAAGTA CCGGAGAGAT ATCAGACCTG AAGTGAAGCT CACTCCCAGA
2101     CACCAGACAT TGTCCACCCT GGAAAGTATA GATGTTCAGG AATTTATTAA GCAAAGACTG
2161     GCAGAAGCAG ACCTAGACCC TAGCGTCCCC CCTTATGACT CTCTTCAGAC TTATGCCTAT
2221     GAGGGTCAGA GATCAGAAGC TGGGTCTATC AGCTCGCTGG ATTCAGCAAC GACACAATCA
2281     GACCAGGATT ATCACTACCT TGGAGACTGG GGACCTGAGT TTAAAAAGTT AGCTGAACTC
2341     TATGGAGAAA TAGAATCTGA AAGAACAACT TAG

 

Quality Control:cDNA sequencing results is available upon request.