Cynomolgus monkey CDH17 Gene cDNA Clone / ORF Clone

CDH17 cDNA Clone Datasheet

CDH17 cDNA Clone Product Information

• cDNA Size: 2499
• Gene Synonym: CDH17
• Source: Cynomolgus monkey

CDH17 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) cadherin 17, LI cadherin (liver-intestine) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 25 T/C, 1622 T/C resulting in the amino acid Ser substitution by Pro, Phe substitution by Ser and 2343 A/G not causing the amino acid variation.
  Please check the sequence information before order.

CDH17 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH17 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH17 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATACTTC AGGCCCATCT TCACCCCCTG TGTCTTCTTA TGCTTTATTT GGCAACTGGA
61       TATGGCCAAG AGGGGAAGTT TAGTGGACCT CTGAAACCCA TGACATTTTC TATTTATGAA
121      GGCCAAGAAC CGAGTCAAAT TATATTCCAG TTTAAGGCCA ATCCTCCTGC TGTGACTTTT
181      GAATTAACTG GGGAGACAGA CAACATATTC AAGATAGAAC GGGAGGGACT TCTGTATTAC
241      ACCAAAGCCT TGGACAGGGA AACAAGATCC ACACACAATC TCCAGGTTGC AGCCCTGGAT
301      GCTAATGGAG CTATAGTGGA GGGTCCAGTC CCCATCACCA TAGAAGTGAA GGACGTCAAT
361      GACAATCGAC CCACATTTCT CCAGTCAAAG TACGAAGGCT CAGTAAGGCA GAACTCTCGC
421      CCAGGAAAGC CCTTCTTGTA CGTCAATGCC ACAGACCTGG ATGATCCGGC CACTCCCAAT
481      GGCCAGCTTT CTTACCAGAT TGTCATCCAG CTTCCTATGA TCAACAATGT CATGTATTTC
541      CAGATCAATA ACAAAACGGG AGGCATCTCT CTTACCCGAG AAGGATCTCA GGAATTGAAC
601      CCTGCTAAGA ATCCTTCCTA TAATCTGGTG ATCTCAGTGA AGGACATGGG AGGCCAGAGT
661      GAGAATTCCT TCAGTGATAC CACATCTGTG GATATCATAG TGACAGAGAA TATTTGGAAA
721      GCACCAGAAC CTGTGGAGAT GGTGGAAAAC TCGACTGATC CTCACCCCAT CAAAATCACT
781      CAGGTGCGGT GGAATGATCC TGGTGCACAA TATTCCTTAG TTGACAAAGA GAAGCTGCCA
841      AGATTCCCAT TTTCAATTGA CCAGGAAGGA GATATTTATG TGACTCAGCC CTTGGACCGA
901      GAAGAAAAGG ATGCATATGT TTTTTATGCA GTTGCAAAGG ATGAGTATGG AAAACCACTT
961      TCATATCCAC TGGAAATTCA TGTAAAAGTT CAAGACATTA ATGATAATCC ACCTACATGT
1021     CCATCACCAG TAACTGTATT TGAGGTCCAG GAGAATGAAC GACTGGGTAA CAGTATTGGG
1081     GCCCTTACTG CACATGACAG CGATGAAGAA AATACTGCCA ACAGTTTGTT GAACTACAGA
1141     ATTGTGGAGC AAACTCCCAA ACTTCCCATG GATGGACTCT TCCTAATCCA AACCTATGCT
1201     GGAATGTTAC AGTTAGCTAA ACAGTCCTTG AAGAAGCAAG ATACTCCTCA GTACAACCTA
1261     ACGATAGAGG TGTCTGACAA AGATTTCAAG ACCCTTTGTT TTGTGCAAAT CAACGTTATT
1321     GATATCAATG ATCAGATCCC CATCTTTGAA AAATCAGATT ATGGAAACCT GACTCTTGCT
1381     GAAGACACAA ACGTTGGGTC CACCATCTTA ACCATCCAGG CCACTGATGC TGATGAGCCA
1441     TTTACTGGGA GTTCTAAAAT TCTGTATCAT GTCATAAAGG GAGACAGTGA GGGACGCCTA
1501     GGGGTTGACA CAGATCCCCA CACCAACACC GGATATGTAA TAATTAAAAA GCCTCTTGAT
1561     TTTGAAACAG CAGCTATTTC CAACATTGTG TTCAAAGCAG AAAATCCTGA GCCTCTAGTG
1621     TCTGGTGTGA CGTACAATGC AAGTTCTTTT GCCAAGTTCA CGCTTTTTGT GACAGATGTG
1681     AATGAAGCAC CTGAATTTTC CCAATACGTA TTCCAAGCGA AAGTCAGTGA GGATGTAGCT
1741     ATAGGCACTA AAGTGGGCAA TGTGACTGCC AAGGATCCAG AAGGTCTGGA CATAAGCTAT
1801     TCACTGAGAG GAGACACAAG AGGTTGGCTT AAAATTGACC ATGTGACTGG TGAGATCTTT
1861     AGTGTGGCTC CATTGGACAG AGAAGCTGGA AGTCCATATC GGGTACAAGT GGTGGCCACT
1921     GAAGTAGGGG GGTCTTCCTT GAGCTCTGTG TCACAGTTCC ACCTGATCCT TACGGATGTG
1981     AATGACAACC CTCCCAGGCT GGCCAAGGAC TACATGGACT TGTACTTCTG CCATCCCCTC
2041     AGTGCACCTG GAAGTCTCAT TTTCGAGGCT ACTGATGATG ATCAGCACTT ATTTCGGGGT
2101     CCCCACTTTA CATTTTCCAT CGCCAGTGAA AGCCTACAGA ACGACTGGCA AGTTTCCAAA
2161     ATCAATGGTA CTCATGCCCG ACTGTCTACC AGGCACACAG ACTTTGAGGA GAAGGAATAT
2221     GTTGTCTCGA TCCGCATCAA TGATGGGGGT CGGCCACCCT TGGAAAGCAC TGTTTCTTTA
2281     ACAGTTACAT TCTGCAGTTG TGGGGAAGAT GGTTGTTTCC GGCCAGCAGG TCACCAGCCT
2341     GGGATACCCA CTGTGGGCAT GGCAGTTGGT ATACTGCTGA CCACTCTTCT GGTCATTGGT
2401     ATAATTTTAG CAGTTGTGTT TATCCGCATG AAGACAGATA AAGGCAAAGA TAATGTTGAA
2461     AGTGCTCAAG CATCTGAAGT CAAACCTCTG AGAAGCTGA

 

Quality Control:cDNA sequencing results is available upon request.