Rat CDH17 Gene cDNA Clone / ORF Clone

CDH17 cDNA Clone Datasheet

CDH17 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_053977.2
• cDNA Size: 2484
• Gene Synonym: Cdh17
• Source: Rat

CDH17 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus cadherin 17 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 957 C/T，39 G/A and 612 G/T not causing the amino acid variation.

CDH17 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH17 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH17 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTCTCTG CCCAGCTTCA CTTCCTGTGT CTTCTCACAC TTTATTTGAC TGGCGCATAT
61       GGCCAAGAAG GGAAGTTCAG CGGCCCCCTG AAACCCATGA CATTCTCTAT TTTTGAAGGC
121      CAAGAACCGA GTCAAATCAT ATTCCAGTTT AAGGCCAACC CTCCAGCTGT GACTTTTGAG
181      CTAACAGGAG AGACGGATGG TATATTTAAG ATAGAAAAGG ATGGACTTCT TTATCACACA
241      AGAGTCCTGG ACAGAGAAAC AAGGGCGGTT CATCATCTGC AGCTTGCAGC CTTGGATTCT
301      CAGGGAGCTA TAGTGGATGG CCCAGTCCCC ATCATCATAG AAGTCAAGGA CATCAATGAC
361      AACCGACCTA CATTTCTCCA GACAAAATAT GAAGGCTCAG TGAGGCAGAA CTCTCGCCCA
421      GGAAAGCCTT TCATGTATGT CAATGCTACA GACCTGGATG ACCCGGCTAC TCCCAATGGC
481      CAGCTTTTTT ATCAAATTGT CATCCAGCTT CCCAAAATCA ACAATGTTAT GTACTTCCAG
541      ATCGACAACA AAACAGGGGC AATCTCGCTT ACCCCAGAAG GTTCTCAGGT ATTGGATCCA
601      ATAAAGAATC CTTACTACAA CCTGGTGGTC TCTGTGAAGG ACATGGGGGG CCAGAATGAG
661      AATTCCTTCA GCGATACCAC ATCTGTGGAT ATTACTGTCA GAGAGAACAT CTGGAAAGCA
721      CCAGAGCCTG TGGAGATTCG AGAAAACTTA ACTGATCCTC ACCCCATCAA AATCACTCAG
781      GTGCAGTGGA ATGACCCAGG GGCCCACTAT TCCTTAATCA ACAAGGAGAA GCTGCCACAG
841      TTTCCATTCT CGATCGACCA AGAAGGAAAT ATTTACGTGA CTCAGCCTTT GGACCGTGAG
901      GAAAAGGACT CTCATGTTTT CTTTGCCACT GCCAAGGATG AGAATGGAAA ACCCCTTGCA
961      TATCCACTGG AAATTCGTGT GAAAGTTATT GACATTAATG ACAACCCACC GACATGTCTC
1021     TCTCAAGTGA CTGTATTTGA AGTCCAGGAG AATGAAGTCT TGGGTAGTAG CATTGGGATC
1081     TTTGCTGCCC ACGATATGGA TGAAGCTAAC AATATCAACA GTATTTTGAA GTACAGACTT
1141     GTAGACCAAA CACCCAAAGT TCCCTCAGAT GAACTCTTTC TCATTGACGA ATATGGAGGA
1201     AAGGTCCAGT TGGGTAAACG GTCCTTGAAG AAGCAAGACA GTCCTCAGTA CAACTTAACA
1261     GTAGAGGTGT CTGACATAGA TTTCAAGACT CTCTGTTCTC TTCAAGTCAA CGTTATTGAT
1321     ATCAACGATC AGATTCCCAT CTTTGAAAGA TCAGACTATG GAAGCAAGAC TCTTTCTGAA
1381     GACACAGCCA TCGGATCCAC CATCTTAATC ATCCAAGCTA CTGATGATGA TGAGCCCTTT
1441     ACCGGGAGCT CTAAAATTCT GTACAAGATT GTACAGGGAG ACACTGAGGG AAGACTGGAA
1501     GTTGTCACAG ATCCCATGAC CAATACAGGA TACGTCAAGA TCAGAAAGCC TCTTGATTTT
1561     GAAACCGAAC CAGTTACTAG CATTGTGTTC AAAGCAGAAA ACCCTGAGCC GCTGGTGAAT
1621     GGCATAGAGT ACAATGCTAG TTCTTTTGCC TCCTTCGAGC TGACCGTGAC GGATGTGAAT
1681     GAAGTGCCTG TATTTCCCCA GCAAATATTC CAAGCCAACG TTAGCGAGGA TACTGCTATA
1741     GGCACTAAAG TGGGCACTGT GACTGCCAGG GATCCGGAAG GCCTGACTGT GAGTTATTCA
1801     CTGAAAGACA ATAAGAGAGG TTGGCTTAAA ATCGACTCCG TCACCGGCGA CATATTTAGC
1861     ACTGCTCCGC TGGACAGGGA AACAGAAAGT GTGTATCGAG TACAAGTGGT GGCCACTGAA
1921     GTAGGTGGGT CCTCTCTGAG TTCTACAGCA TATTTCCACC TGGTCCTCAT GGATGTGAAT
1981     GACAACCCCC CTCGCCTAGC TAAGGACTAC ACAGGCTTGT TCTTCTGCCA TCCCCTAAGT
2041     GCCCCTGGGA GCCTCATCTT TGAAGCCACT GATGATGACC AGCAGTCAGT TCGGAGGCCC
2101     AAGTTTACGT TTGCCCTTGG CAGAGAAAGC TTACAAAGTG ACTGGGAAGT GTCCAAAATC
2161     AATGGCACAC ATGCTAGACT CTCCACCAAG CACACACGCT TTGAGGAACA AGTTTATGAC
2221     ATCCCAATCC TCATCAATGA TGGGGGGCAG CCACCCATGG AAGGCATTGT CTCCTTATCA
2281     GTTACTTTCT GCCAGTGTGT GGACGGAAGT TGTTTCCGGC CAGCAGGCAA CCAGGTTGGG
2341     ATACCCACCG TGGGCATGGC AGTTGGTATA CTTCTGACCA CATTTCTGGT CATTGGTATA
2401     ATTTTAGCGG TTGTATTCAT CCGTATGAGG AAAGATAAAG TTGAAGATCC ACAGTCCCCT
2461     GAGAATAAGC CTCTGAGGAG CTGA

 

Quality Control:cDNA sequencing results is available upon request.