|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus cadherin 16 DNA.|
|Identical with the Gene Bank Ref. ID sequence except for the point mutations: 455 T/C resulting in the amino acid Val substitution by Ala and 1086 A/G,1671 G/A not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat CDH16 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80282-G-F|
|Rat CDH16 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80282-G-H|
|Rat CDH16 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80282-G-M|
|Rat CDH16 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80282-G-N|
|Rat CDH16 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80282-G-Y|
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KSP-Cadherin/Cadherin-16 is a member of the cadherin superfamily, calcium-dependent, membrane-associated glycoproteins. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. KSP-Cadherin/Cadherin-16 can be detected at later stages of tubulogenesis during human renal development and in the distal tubules of adult kidneys, no expression was found by immunohistochemistry or Western blot analysis in RCC tumour tissues and several RCC cell lines. However, despite the lack of protein expression, mRNA synthesis of KSP-Cadherin/Cadherin-16 could be detected by reverse transcriptase-polymerase chain reaction analysis in all RCC tissues and most of the RCC cell lines studied, although at a reduced level. The loss of KSP-Cadherin/Cadherin-16 protein was only observed in the malignant part of the tumour kidneys, whereas in the normal part of the affected kidneys KSP-Cadherin/Cadherin-16 expression was clearly detected. These results indicate a downregulation of Ksp-cadherin in RCC and suggest a role for this cell adhesion molecule in tumour suppression.