Rat CDH13 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80280-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CDH13 cDNA Clone Datasheet

CDH13 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_138889.2
cDNA Size:	2145
Gene Synonym:	Cdht; Tcad; MGC93172; T-cadherin; Cdh13
Source:	Rat

CDH13 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus cadherin 13 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1285 C/T resulting in the amino acid Pro substitution by Ser and 249 T/C，1386 C/A not causing the amino acid variation.

CDH13 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH13 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH13 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGCCGA GAACTCCGCT CACCCTGTGC GTCCTGCTGT CCCAGGTGCT CCTGGTAACA
61       TCTGCAGGTG ATTTGGACTG CACCCCCGGA TTCCAGCAGA AAGTGCTACA CATCCACCAG
121      CCTGCCGAAT TCATCGAGGA CCAGCCGGTC CTAAACTTGA CCTTCAGTGA CTGCAAGGGC
181      AACAAGAAGC TGCACTATGA GGTCTCAAGC CCATACTTCA AGGTGAACAG CGATGGCACC
241      TTAGTGGCCG TCAGAAACAT CACTGCGGTG GGCAGGACCC TGTTTGTCCA CGCAAGGACT
301      CCTCATGCCG AAGACATGGC AGAACTCGTG ATTGTCGGGG GGAAAGACAT CCAGGGCTCC
361      TTACAGGACA TTTTTAAATT TGCAAGGACA TCTCCTGTCC CAAGACAAAA GAGGTCCATT
421      GTGGTGTCCC CCATCTTGAT CCCAGAGAAC CAGAGACAGC CCTTCCCCAG AGATGTTGGC
481      AAGGTCGTTG ATAGTGACAG ACCCGAAGGG TCCAAGTTCC GGCTCACGGG GAAGGGAGTG
541      GATCAAGACC CTAAAGGGAC TTTCAGAATC AATGAGAACA CGGGAAGCGT CTCCGTGACA
601      CGGACCCTGG ACAGAGAAAC GATCGCTACT TATCAACTGT TTGTGGAGAC CACAGATGCC
661      AGTGGCAGAA CTCTGGAGGG ACCAGTGCCT CTGGAAGTCA TCGTGATTGA TCAGAATGAC
721      AACCGACCCA TCTTCCGGGA AGGCCCTTAC ATCGGCCATG TCATGGAAGG GTCACCCACA
781      GGGACCACGG TGATGCGGAT GACAGCGTTT GATGCAGATG ACCCGGCCAC TGACAACGCT
841      CTCCTGCGGT ACAACATCCG TCAGCAGACG CCTGACAAGC CGTCTCCTAA CATGTTCTAC
901      ATCGATCCCG AGAAAGGAGA CATTGTCACC GTGGTGTCAC CTGCGCTGCT GGACCGGGAG
961      ACTCTGGAAA ACCCCAAGTA TGAACTGATC ATCGAAGCTC AAGATATGGC AGGACTGGAC
1021     GTTGGATTGA CAGGCACGGC CACAGCCACC ATTGTGATCG ATGACAAAAA TGATCACTCA
1081     CCAAAATTCA CCAAGAAAGA GTTTCAAGCC ACAGTGGAGG AGGGAGCCGT GGGAGTCATT
1141     GTCAACTTGA CAGTGGAAGA CAAAGATGAC CCCGCGACAG GAGCATGGAG GGCGGCATAC
1201     ACCATCATCA ATGGAAATCC TGGGCAGAGC TTTGAGATCC ACACCAACCC ACAGACCAAC
1261     GAGGGCATGC TCTCTGTGGT CAAGTCCCTG GACTATGAGA TCTCTGCCTT TCACACCCTG
1321     CTGATCAAAG TGGAGAATGA GGACCCATTG GTACCCGACG TCTCCTATGG CCCCAGCTCC
1381     ACGGCAACTG TCCACATCAC GGTCTTGGAT GTCAACGAGG GACCAGTCTT CTACCCAGAC
1441     CCCATGATGG TGACCAAACA GGAAAATATC TCTGTGGGCA GTGTGCTGCT GACAGTGAAT
1501     GCCACAGACC CGGACTCCCT GCAACATCAA ACTATCAGGT ACTCTGTTTA CAAGGACCCA
1561     GCGGGCTGGC TGAGTATTAA CCCCATCAAT GGCACTGTCG ACACCACAGC TGTGTTGGAC
1621     CGGGAGTCTC CATTCGTCCA CAACAGTGTG TACACTGCCC TCTTCCTGGC CATCGACAGC
1681     GGCAACCCTC CTGCGACTGG CACCGGGACT CTGATGATCA CCCTGGAGGA CGTTAACGAC
1741     AACGCTCCTG TCATTTACCC AACGGTGGCT GAAGTCTGCG ACGACGCCAG AAACCTCAGC
1801     GTGGTCATCC TGGGGGCTTC AGACAAGGAC CTTCACCCCA ACACAGACCC CTTCAAGTTC
1861     GAGATTCATA AACAGACCGT CCCTGATAAA GTCTGGAAGA TCTCCAAAAT CAACAACACC
1921     CACGCCCTCG TGAGCCTTCT TCAGAATCTG AACAAGGCGA ACTACAACCT GCCCATCATG
1981     GTGACGGATT CAGGGAAGCC ACCCATGACG AACATCACGG ACCTCAGGGT GCAGGTGTGC
2041     TCTTGCAAGA ACTCCAAAGT GGACTGCAAT GCGGCGGGGA CCCTGCACCT CAGCCTCAGC
2101     TTGCTGCTGC TCTTCTCTCT CCTCAGCTTA TTGTCAGGTC TGTGA

Quality Control:cDNA sequencing results is available upon request.