Rat CDH12 Gene cDNA Clone / ORF Clone

CDH12 cDNA Clone Datasheet

CDH12 cDNA Clone Product Information

• Gene Bank Ref.ID: XM_229369.5
• cDNA Size: 2385
• Gene Synonym: RGD1566350; Cdh12
• Source: Rat

CDH12 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus cadherin 12 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 871-873 ATA/GAT resulting in the amino acid Ile substitution by Asp and 207 A/C,1083 A/G，1542 T/C，1806 C/T，2265 C/T not causing the amino acid variation.

CDH12 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH12 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH12 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTTACAA GGAACTCTTT ATACCTGCTT CTCTGGATCC TGTTTGATGG AGGTCTCCTA
61       ACACCACTGC AGCCACAGCC ACAACAAACT TTAGCCACAG AACCAAAAGA CAATGTTATC
121      CACCTTCTGG GGAGACGGTC CCATTTCCAA CGAGTTAAAC GTGGCTGGGT ATGGAATCAG
181      TTTTTTGTGC TGGAAGAGTA CATGGGCTCC GAACCTCAAT ATGTGGGAAA GTTACACTCT
241      GACCTGGACA AGGGAGAAGG CACTGTTAAA TACACGCTCT CTGGAGATGG TGCTGGCACT
301      GTTTTCACGA TTGATGAAAC TACAGGAGAC ATTCATGCAA TAAGAAGCCT GGATAGAGAA
361      GAAAAACCTT TCTACACTCT TCGTGCTCAG GCAGTGGACA TAGAAACCAG GAAGCCACTG
421      GAGCCCGAAT CAGAGTTCAT CATTAAAGTG CAGGATATTA ACGATAATGA GCCAAAGTTT
481      TTGGATGGAC CATATGTTGC TAGTGTTCCA GAAATGTCTC CTGTGGGTGC ATATGTGCTA
541      CAGGTCAAGG CCACAGATGC AGATGACCCC ACCTATGGAA ACAGTGCCAG AGTTGTTTAC
601      AGCATCCTTC AGGGGCAGCC TTATTTCTCC ATTGATCCCA AAACAGGTGT CATTAGAACA
661      GCATTGCCAA ACATGGACAG AGAAGTCAAG GAGCAGTACC AGGTCCTCAT TCAAGCGAAG
721      GACATGGGAG GACAGCTCGG AGGACTCGCT GGAACTACCA TTGTCAACAT CACCCTTACT
781      GATGTCAATG ACAACCCACC TCGATTCCCA AAAAGCATCT TCCATCTAAA AGTTCCCGAG
841      TCCTCCCCTG TTGGCTCAGC TATTGGAAGA GATAGAGCAG TAGATCCTGA TTTTGGAAAA
901      AATGCAGAAA TTGAATACAA CATTGTCCCA GGAGATGGGG GAAATTTGTT TGACATTGTC
961      ACAGATGAGG ATACACAAGA AGGAATCATC AAATTGAAAA AGCCATTAGA TTTTGAAACA
1021     AAGAAGGCAT ATACTTTTAA AGTAGAGGCC TCCAACCTTC ACCTTGACCA CCGCTTTCAC
1081     TCGGCTGGGC CATTTAAAGA TACTGCTACG GTAAAAATAA GCGTGCTGGA TGTGGACGAG
1141     CCGCCAGTGT TCAGCAAGCC ACTTTACACC ATGGAGGTTT ATGAAGACAC TCCTGTGGGG
1201     ACCATCATCG GAGCTGTCAC AGCACAAGAC CTTGATGTGG GCAGTAGTGC TGTTAGGTAC
1261     TTCATAGATT GGAAGAGTGA TGGGGACAGC TACTTCACAA TAGATGGAAC GGAAGGAACC
1321     ATCGCCACTA ATGAATTACT AGACAGAGAG AACACAGCAC AGTATAATTT CTCAATAATT
1381     GCAAGTAAAG TTAGTAACCC ATCATTAACT AGCAAAGTTA ATATATTAAT TAATGTCCTA
1441     GATGTCAATG AATTTCCGCC AGAAATATCT GTGCCATATG AGACAGCTGT GTGTGAAAAT
1501     GCCAAACCAG GACAGATCAT TCAGATAGTC GGTGCTGTAG ACCGAGATCT CTCACCTGCT
1561     GGGCAGCAAT TCTCCTTTAG ATTAGCACCA GAAGCTGTCA TCAAACCAAA TTTTACAGTT
1621     CATGACTTCA GAAACAACAC AGCTGGAATC GAAACCCGGA GAAATGGCTA CAGCCGCAGG
1681     CAACAAGAAT TGTATTTCCT TCCTATTGTT ATAGAAGACA GCAGTTACCC TGTCCAAAGC
1741     AGCACGAATA CAATGACAAT TCGTGTTTGT AGATGTGACT CTGATGGAAC CATCCTGTCT
1801     TGCAATGTGG AAGCAATTTT TCTACCTGTA GGACTTAGCA CCGGAGCTTT GATTGCAATC
1861     CTACTATGCA TTGTTATACT CTTAGCTATT GTTGTGCTGT ATGTAGCACT GAGGAGGCAG
1921     AAGAAGAAAG ACACCCTTAT GACCTCCAAA GAAGACATCA GAGACAATGT TATCCATTAT
1981     GATGATGAAG GAGGTGGGGA GGAGGACACC CAAGCCTTTG ACATTGGGGC TCTGAGAAAT
2041     CCGAAGGTGA TCGAAGAGAA CAAAATTCGC AGAGATATAA AACCAGACTC CTTCTGTTTA
2101     CCTCGTCAGA GACCACAAGT GGAAGACAAC ACAGACATAA GGGATTTTAT TCACCAAAGG
2161     CTACAGGAAA ATGATGCAGA TCCAGCTGCA CCCCCATATG ATTCCTTGGC CACTTATGCT
2221     TATGAGGGGA ATGGGTCAGT GGCTGAGTCA CTCAGCTCCA TAGATTCTCT CACCTCAGAG
2281     GCTGACCAGG ACTATGATTA TCTATCAGAC TGGGGACCCC GCTTTAAGGT CTTGGCAGAC
2341     ATGTTTGGAG AAGAAGAGAG TTACAACCCT GACAATGTTA CCTAG

 

Quality Control:cDNA sequencing results is available upon request.