Human CDH11 ORF Expression Clone, FLAG-tagged

CDH11 cDNA Clone Datasheet

CDH11 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001797.2
• cDNA Size: 2418
• Gene Synonym: CDH11, OB, CAD11, CDHOB, OSF-4
• Source: Human

CDH11 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens cadherin 11, type 2, OB-cadherin (osteoblast) DNA.
• Vector: pCMV/hygro-FLAG
• FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 825 G/A and 1117 T/G resulting in the amino acid MET substitution by ILE and ser substitution by ala.

CDH11 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH11 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH11 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGAGA ACTACTGTTT ACAAGCCGCC CTGGTGTGCC TGGGCATGCT GTGCCACAGC
61       CATGCCTTTG CCCCAGAGCG GCGGGGGCAC CTGCGGCCCT CCTTCCATGG GCACCATGAG
121      AAGGGCAAGG AGGGGCAGGT GCTACAGCGC TCCAAGCGTG GCTGGGTCTG GAACCAGTTC
181      TTCGTGATAG AGGAGTACAC CGGGCCTGAC CCCGTGCTTG TGGGCAGGCT TCATTCAGAT
241      ATTGACTCTG GTGATGGGAA CATTAAATAC ATTCTCTCAG GGGAAGGAGC TGGAACCATT
301      TTTGTGATTG ATGACAAATC AGGGAACATT CATGCCACCA AGACGTTGGA TCGAGAAGAG
361      AGAGCCCAGT ACACGTTGAT GGCTCAGGCG GTGGACAGGG ACACCAATCG GCCACTGGAG
421      CCACCGTCGG AATTCATTGT CAAGGTCCAG GACATTAATG ACAACCCTCC GGAGTTCCTG
481      CACGAGACCT ATCATGCCAA CGTGCCTGAG AGGTCCAATG TGGGAACGTC AGTAATCCAG
541      GTGACAGCTT CAGATGCAGA TGACCCCACT TATGGAAATA GCGCCAAGTT AGTGTACAGT
601      ATCCTCGAAG GACAACCCTA TTTTTCGGTG GAAGCACAGA CAGGTATCAT CAGAACAGCC
661      CTACCCAACA TGGACAGGGA GGCCAAGGAG GAGTACCACG TGGTGATCCA GGCCAAGGAC
721      ATGGGTGGAC ATATGGGCGG ACTCTCAGGG ACAACCAAAG TGACGATCAC ACTGACCGAT
781      GTCAATGACA ACCCACCAAA GTTTCCGCAG AGCGTATACC AGATATCTGT GTCAGAAGCA
841      GCCGTCCCTG GGGAGGAAGT AGGAAGAGTG AAAGCTAAAG ATCCAGACAT TGGAGAAAAT
901      GGCTTAGTCA CATACAATAT TGTTGATGGA GATGGTATGG AATCGTTTGA AATCACAACG
961      GACTATGAAA CACAGGAGGG GGTGATAAAG CTGAAAAAGC CTGTAGATTT TGAAACCAAA
1021     AGAGCCTATA GCTTGAAGGT AGAGGCAGCC AACGTGCACA TCGACCCGAA GTTTATCAGC
1081     AATGGCCCTT TCAAGGACAC TGTGACCGTC AAGATCGCAG TAGAAGATGC TGATGAGCCC
1141     CCTATGTTCT TGGCCCCAAG TTACATCCAC GAAGTCCAAG AAAATGCAGC TGCTGGCACC
1201     GTGGTTGGGA GAGTGCATGC CAAAGACCCT GATGCTGCCA ACAGCCCGAT AAGGTATTCC
1261     ATCGATCGTC ACACTGACCT CGACAGATTT TTCACTATTA ATCCAGAGGA TGGTTTTATT
1321     AAAACTACAA AACCTCTGGA TAGAGAGGAA ACAGCCTGGC TCAACATCAC TGTCTTTGCA
1381     GCAGAAATCC ACAATCGGCA TCAGGAAGCC AAAGTCCCAG TGGCCATTAG GGTCCTTGAT
1441     GTCAACGATA ATGCTCCCAA GTTTGCTGCC CCTTATGAAG GTTTCATCTG TGAGAGTGAT
1501     CAGACCAAGC CACTTTCCAA CCAGCCAATT GTTACAATTA GTGCAGATGA CAAGGATGAC
1561     ACGGCCAATG GACCAAGATT TATCTTCAGC CTACCCCCTG AAATCATTCA CAATCCAAAT
1621     TTCACAGTCA GAGACAACCG AGATAACACA GCAGGCGTGT ACGCCCGGCG TGGAGGGTTC
1681     AGTCGGCAGA AGCAGGACTT GTACCTTCTG CCCATAGTGA TCAGCGATGG CGGCATCCCG
1741     CCCATGAGTA GCACCAACAC CCTCACCATC AAAGTCTGCG GGTGCGACGT GAACGGGGCA
1801     CTGCTCTCCT GCAACGCAGA GGCCTACATT CTGAACGCCG GCCTGAGCAC AGGCGCCCTG
1861     ATCGCCATCC TCGCCTGCAT CGTCATTCTC CTGGTCATTG TAGTATTGTT TGTGACCCTG
1921     AGAAGGCAAA AGAAAGAACC ACTCATTGTC TTTGAGGAAG AAGATGTCCG TGAGAACATC
1981     ATTACTTATG ATGATGAAGG GGGTGGGGAA GAAGACACAG AAGCCTTTGA TATTGCCACC
2041     CTCCAGAATC CTGATGGTAT CAATGGATTT ATCCCCCGCA AAGACATCAA ACCTGAGTAT
2101     CAGTACATGC CTAGACCTGG GCTCCGGCCA GCGCCCAACA GCGTGGATGT CGATGACTTC
2161     ATCAACACGA GAATACAGGA GGCAGACAAT GACCCCACGG CTCCTCCTTA TGACTCCATT
2221     CAAATCTACG GTTATGAAGG CAGGGGCTCA GTGGCCGGGT CCCTGAGCTC CCTAGAGTCG
2281     GCCACCACAG ATTCAGACTT GGACTATGAT TATCTACAGA ACTGGGGACC TCGTTTTAAG
2341     AAACTAGCAG ATTTGTATGG TTCCAAAGAC ACTTTTGATG ACGATTCTGC T1GATTACAAG
2401     GATGACGACG ATAAG2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

 

 

Quality Control:cDNA sequencing results is available upon request.