Rat CDH1 Gene cDNA Clone / ORF Clone

CDH1 cDNA Clone Datasheet

CDH1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031334.1
• cDNA Size: 2661
• Gene Synonym: Cdh1
• Source: Rat

CDH1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus cadherin 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 195 C/T not causing the amino acid variation.

CDH1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGCCC GGTGCCGCAG CTTCTCCGCG CTCCTGCTCC TGCTGCAGGT CTCCTCGTGG
61       CTTTGTCAGC AGCCGGAGTC GGAGTCTGAC TCCTGCCGTC CCGGCTTCAG TTCCGAGGTC
121      TACACCTTCC TGGTGCCGGA GAGGCACCTG GAGAGAGGCC ACATCCTGGG CAGAGTGAAA
181      TTTGAAGGAT GCACTGGCCG TCCAAGGACA GCCTTCTTTT CTGAAGACTC CCGATTCAAA
241      GTGTCTACAG ATGGCGTCAT CACAGTCAAA CGGCATCTAA AGCTTCACAA GCTGGAGACC
301      AGTTTTCTCG TCCATGCCTG GGACTCCAGT TACAGGAAGC TTTCTACCAA AGTGACACTG
361      AAGTCCCTGG GCCACCACCA CCACCGGCAT CACCACAGAG ACCCTGTCTC TGAATCCAAC
421      CCAGAGCTGC TCACGTTTCC CAGCTTTCAC CAGGGTCTGA GAAGACAGAA ACGAGACTGG
481      GTCATCCCTC CCATCAACTG CCCGGAAAAT CAAAAGGGCG AATTCCCCCA GCGACTGGTT
541      CAGATCAAAT CCAACAGGGA CAAAGAGACA ACGGTTTTCT ACAGCATCAC CGGCCCAGGA
601      GCTGACAAAC CCCCTGTTGG CGTTTTCATC ATTGAGAGGG AGACAGGCTG GCTGAAAGTG
661      ACGCAGCCTC TGGACAGAGA AGCCATTGAC AAGTACCTTC TCTACTCTCA TGCTGTGTCA
721      TCAAATGGGG AAGCCGTGGA GGATCCCATG GAGATAGTGG TCACAGTCAC AGATCAGAAT
781      GACAACAGGC CAGAGTTTAT CCAGGAGGTC TTTGAGGGAT CTGTTGCAGA AGGCGCTCTT
841      CCAGGAACCT CCGTGATGCA GGTCTCAGCC ACTGATGCAG ACGATGACAT AAACACCTAC
901      AATGCTGCCA TCGCCTACAC CATCCTCAGC CAAGATCCTG AGCTGCCTCA CAAAAACATG
961      TTCACTGTCA ACCGGGACAC TGGGGTCATC AGTGTGGTCA CCTCCGGACT GGACCGAGAG
1021     AGTTACCCTA CATATACTCT GGTGGTTCAG GCTGCCGACC TTCAAGGCGA AGGCCTAAGC
1081     ACAACAGCAA AAGCTGTGAT CACTGTCAAG GATATTAATG ACAACGCTCC CATCTTCAAC
1141     CCAAGCACGT ACCAGGGTCA AGTGCTTGAG AATGAGGTCG GTGCCCGTAT TGCCACACTC
1201     AAGGTGACTG ATGATGATGC CCCCAACACT CCAGCGTGGA ATGCTGTGTA CACCGTAGTC
1261     AATGATCCTG ATCATCAGTT CACTGTCATC ACAGACCCCA AGACCAACGA GGGCATTCTG
1321     AAAACAGCCA AGGGCTTGGA TTTTGAGGCC AAGCAGCAGT ACATTCTGCA CGTGACAGTG
1381     GAAAATGAGG AGCCCTTTGA GGGGTCTCTT GTCCCTTCCA CAGCCACTGT CACCGTGGAT
1441     GTGGTAGACG TGAATGAAGC CCCCATTTTT GTGCCTGCGG AGAAGAGAGT CGAGGTGCCT
1501     GAGGACTTTG GTGTGGGTCT GGAGATCGCA TCTTACACTG CGCGAGAGCC AGACACATTC
1561     ATGGAACAGA AGATCACGTA TCGGATTTGG AGGGACACTG CCAATTGGCT GGAGATTAAC
1621     CCAGAGACTG GGGTCATTTC CACTCGGGCT GAGATGGACA GAGAAGATTC GGAGCATGTG
1681     AAGAACAGCA CGTATACAGC TCTCATCATT GCCACAGATG ATGGTTCACC CATTGCCACT
1741     GGCACAGGGA CTCTTCTCCT GGTCCTGTCA GACGTCAACG ACAATGCTCC CATCCCAGAA
1801     CCTCGAAATA TGCAGTTCTG CCAGAGAAAC CCGAAGCCCC ATGTCATCAC CATCTTGGAT
1861     CCAGACCTTC CCCCAAACAC ATCCCCCTTC ACTGCAGAGC TCACCCATGG GGCCAGCGTC
1921     AACTGGACCA TTGAGTACAA TGACGCAGAA CAAGAATCTC TCATTTTGCA ACCAAGAAAG
1981     GACTTAGAGA TTGGCGAATA CAAAATCAAT CTCAAGCTCT CGGATAACCA GAATAAAGAC
2041     CAGGTGACCA CGTTGGAGGT CCACGTGTGT GACTGTGAAG GGACCGTCAA CAACTGCATG
2101     AAGGCGATCT CCCTGGAAGC AGGATTACAA GTTCCCGCCA TCCTTGGAAT CCTGGGAGGG
2161     ATCCTGGCCC TCCTGATTCT GATCCTCCTG CTCCTACTGT TTCTACGGAG GAGAACGGTG
2221     GTCAAAGAGC CCTTGCTGCC ACCAGATGAC GATACCCGGG ACAATGTGTA TTACTATGAT
2281     GAAGAGGGAG GTGGAGAAGA AGACCAGGAC TTTGATTTGA GCCAGCTGCA CAGGGGCCTT
2341     GATGCCAGAC CGGAAGTGAT TCGAAATGAT GTGGCTCCCA CCCTCATGAG CATGCCCCAG
2401     TATCGTCCCC GTCCAGCCAA TCCTGATGAA ATCGGGAACT TCATCGATGA AAACCTGAAG
2461     GCAGCGGACA GTGACCCCAC AGCGCCCCCT TACGACTCTC TGTTGGTGTT TGACTATGAG
2521     GGGAGTGGTT CTGAAGCTGC CTCCCTGAGC TCGCTGAACT CCTCTGAGTC AGATCAGGAC
2581     CAGGACTACG ATTATCTGAA CGAATGGGGC AACCGGTTCA AGAAGCTGGC CGATATGTAT
2641     GGTGGCGGCG AAGAAGACTA G

 

Quality Control:cDNA sequencing results is available upon request.