Gene Summary: This CDC42BPB gene encodes a member of the serine/threonine protein kinase family. CDC42BPB contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of CDC42BPB is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]General information above from NCBI
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium (By similarity).
Enzyme regulation: Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation (By similarity). Inhibited by chelerythrine chloride.
Subunit structure: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42. Interacts with TJP1, when in the presence of catalytically active CDC42 (By similarity). Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPB and MYO18A. LURAP1 binding results in activation of CDC42BPB by abolition of its negative autoregulation.
Subcellular location: Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell- cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42.
Tissue specificity: Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
Sequence similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 CNH domain.
Contains 1 CRIB domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
General information above from UniProt