Human CDC25C Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12323-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CDC25C cDNA Clone Datasheet

CDC25C cDNA Clone Product Information

Gene Bank Ref.ID:	BC019089
cDNA Size:	1422
Gene Synonym:	CDC25; PPP1R60；CDC25C
Source:	Human

CDC25C cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens cell division cycle 25 homolog C (S. pombe) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CDC25C cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDC25C cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDC25C cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTACGG AACTCTTCTC ATCCACAAGA GAGGAAGGAA GCTCTGGCTC AGGACCCAGT
61       TTTAGGTCTA ATCAAAGGAA AATGTTAAAC CTGCTCCTGG AGAGAGACAC TTCCTTTACC
121      GTCTGTCCAG ATGTCCCTAG AACTCCAGTG GGCAAATTTC TTGGTGATTC TGCAAACCTA
181      AGCATTTTGT CTGGAGGAAC CCCAAAATGT TGCCTCGATC TTTCGAATCT TAGCAGTGGG
241      GAGATAACTG CCACTCAGCT TACCACTTCT GCAGACCTTG ATGAAACTGG TCACCTGGAT
301      TCTTCAGGAC TTCAGGAAGT GCATTTAGCT GGGATGAATC ATGACCAGCA CCTAATGAAA
361      TGTAGCCCAG CACAGCTTCT TTGTAGCACT CCGAATGGTT TGGACCGTGG CCATAGAAAG
421      AGAGATGCAA TGTGTAGTTC ATCTGCAAAT AAAGAAAATG ACAATGGAAA CTTGGTGGAC
481      AGTGAAATGA AATATTTGGG CAGTCCCATT ACTACTGTTC CAAAATTGGA TAAAAATCCA
541      AACCTAGGAG AAGACCAGGC AGAAGAGATT TCAGATGAAT TAATGGAGTT TTCCCTGAAA
601      GATCAAGAAG CAAAGGTGAG CAGAAGTGGC CTATATCGCT CCCCGTCGAT GCCAGAGAAC
661      TTGAACAGGC CAAGACTGAA GCAGGTGGAA AAATTCAAGG ACAACACAAT ACCAGATAAA
721      GTTAAAAAAA AGTATTTTTC TGGCCAAGGA AAGCTCAGGA AGGGCTTATG TTTAAAGAAG
781      ACAGTCTCTC TGTGTGACAT TACTATCACT CAGATGCTGG AGGAAGATTC TAACCAGGGG
841      CACCTGATTG GTGATTTTTC CAAGGTATGT GCGCTGCCAA CCGTGTCAGG GAAACACCAA
901      GATCTGAAGT ATGTCAACCC AGAAACAGTG GCTGCCTTAC TGTCGGGGAA GTTCCAGGGT
961      CTGATTGAGA AGTTTTATGT CATTGATTGT CGCTATCCAT ATGAGTATCT GGGAGGACAC
1021     ATCCAGGGAG CCTTAAACTT ATATAGTCAG GAAGAACTGT TTAACTTCTT TCTGAAGAAG
1081     CCCATCGTCC CTTTGGACAC CCAGAAGAGA ATAATCATCG TGTTCCACTG TGAATTCTCC
1141     TCAGAGAGGG GCCCCCGAAT GTGCCGCTGT CTGCGTGAAG AGGACAGGTC TCTGAACCAG
1201     TATCCTGCAT TGTACTACCC AGAGCTATAT ATCCTTAAAG GCGGCTACAG AGACTTCTTT
1261     CCAGAATATA TGGAACTGTG TGAACCACAG AGCTACTGCC CTATGCATCA TCAGGACCAC
1321     AAGACTGAGT TGCTGAGGTG TCGAAGCCAG AGCAAAGTGC AGGAAGGGGA GCGGCAGCTG
1381     CGGGAGCAGA TTGCCCTTCT GGTGAAGGAC ATGAGCCCAT GA

Quality Control:cDNA sequencing results is available upon request.