Mouse CDK1/CDC2 Gene cDNA Clone / ORF Clone

CDK1/CDC2 cDNA Clone Datasheet

CDK1/CDC2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007659.3
• cDNA Size: 894
• Gene Synonym: Cdc2; Cdc2a; p34<CDC2>; Cdk1
• Source: Mouse

CDK1/CDC2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cyclin-dependent kinase 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CDK1/CDC2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDK1/CDC2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDK1/CDC2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAAGACT ATATCAAAAT AGAGAAAATT GGAGAAGGTA CTTACGGTGT GGTGTATAAG
61       GGTAGACACA GAGTCACTGG CCAGATAGTG GCCATGAAGA AGATCAGACT TGAAAGCGAG
121      GAAGAAGGAG TGCCCAGTAC TGCAATTCGG GAAATCTCTC TATTAAAAGA ACTTCGACAT
181      CCAAATATAG TCAGCCTGCA GGATGTGCTC ATGCAGGACT CCAGGCTGTA TCTCATCTTT
241      GAGTTCCTGT CCATGGACCT CAAGAAGTAC CTGGACTCCA TCCCTCCTGG GCAGTTCATG
301      GATTCTTCAC TCGTTAAGAG TTACTTACAC CAAATCCTCC AGGGAATTGT GTTTTGCCAC
361      TCCCGGCGAG TTCTTCACAG AGACTTGAAA CCTCAAAATC TATTGATTGA TGACAAAGGA
421      ACAATCAAAC TGGCTGATTT CGGCCTTGCC AGAGCGTTTG GAATACCGAT ACGAGTGTAC
481      ACACACGAGG TAGTGACGCT GTGGTACCGA TCTCCAGAAG TGTTGCTGGG CTCGGCTCGT
541      TACTCCACTC CGGTTGACAT CTGGAGTATA GGGACCATAT TTGCAGAACT GGCCACCAAG
601      AAGCCGCTTT TCCACGGCGA CTCAGAGATT GACCAGCTCT TCAGGATCTT CAGAGCTCTG
661      GGCACTCCTA ACAACGAAGT GTGGCCAGAA GTCGAGTCCC TGCAGGACTA CAAGAACACC
721      TTTCCCAAGT GGAAGCCGGG GAGCCTCGCA TCCCACGTCA AGAACCTGGA CGAGAACGGC
781      TTGGATTTGC TCTCAAAAAT GCTAGTCTAT GATCCTGCCA AACGAATCTC TGGCAAAATG
841      GCCCTGAAGC ACCCGTACTT TGATGACTTG GACAATCAGA TTAAGAAGAT GTAG

 

Quality Control:cDNA sequencing results is available upon request.