Rat CD99 Gene cDNA Clone / ORF Clone

CD99 cDNA Clone Datasheet

CD99 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001100804.1
• cDNA Size: 498
• Gene Synonym: Cd99
• Source: Rat

CD99 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus CD99 antigen DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD99 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD99 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD99 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCGCG CGGCGGGAAT CACAGCGGCG ATAACCTTGG CGCTACTCGG GGTCCTGGCG
61       CTCGGCGCAG GTGATGGTGA CTTCAGACTG GACGATGCCC TGGAGGACAC TGACAAGAAG
121      CCAACGCCCA AGCCCCCGAC ACCCAAGAAG CCATCCTCAG GAGACTTTGA CCTGGAGGAG
181      GCACTGACTG GCGGTGCTGA TGAGGATCCC CGGAGGCCAG GAAGCCGGCC CAAGCCTGAT
241      CCGAAGCCAC CTGGGCCACC CAGAGACTCA GGAGGAATCT CTGACCGAGA CCTGGAGGAT
301      GTGGCTGGAC ATGGGGGACG AGGAGGCGGA GCGGGGGACC GCGGTACTGA TGGGGCGGAG
361      TCGGAAGGCC AGCCCCAGGG CCTAATCCCA GGTGTGGTCG CTGCAGTCCT GGCGGCGCTC
421      GCGGGGGCTG TGTCCAGCTT TGTAGCCTAT CAGAAGCGGC GCCTGTGCTT TCGGGAAGGC
481      GGCTCCGCCC CCGTCTAG

 

Quality Control:cDNA sequencing results is available upon request.