Rat CD99 Protein

CD99 antigen Protein Datasheet

CD99 Protein Product Information

• Synonym: CD99
• Protein Construction: A DNA sequence encoding the rat CD99 (B4F7A5) (Met1-Gly127) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD99 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 25
• Molecular Mass: The recombinant rat CD99 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 344 amino acids and has a predicted molecular mass of 37.6 kDa. The apparent molecular mass of the protein is approximately 47 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD99 protein

CD99 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD99 Protein Description

CD99 antigen, also known as T-cell surface glycoprotein E2, E2 antigen, Protein MIC2 and CD99, is a single-pass type I  membrane protein which belongs to the CD99 family. CD99 is characteristically expressed in Ewing's sarcoma/primitive neuroectodermal tumor. CD99 down-regulation may have a role in osteosarcoma development and progression. CD99-forced expression in two osteosarcoma cell lines significantly reduced resistance to anoikis, inhibited growth in anchorage independence as well as cell migration, and led to abrogation of tumorigenic and metastatic ability. CD99 acts as a potent suppressor of malignancy in osteosarcoma. CD99 is involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. Expression of CD99 on the surface of circulating polymorphonuclear cells (PMN) is low compared with expression of CD99 on monocytes or expression of PECAM on PMN. PECAM (CD31) and CD99 regulate distinct, sequential steps in the transendothelial migration of neutrophils during inflammation. CD99 signal transduction can also deliver effective costimulatory signals to T cells.

References

1. Wingett D. et al., 1999, Cell Immunol. 193 (1): 17-23.
2. Jung KC. et al., 2002, J Korean Med Sci. 17 (4): 483-9.
3. Manara MC. et al., 2006, Mol Biol Cell. 17 (4): 1910-21.
4. Wilkerson AE. et al., 2006,  J Cutan Pathol. 33 (10): 663-6.