Anti-CD97 Antibody

Cat: 11280-R024
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All CD97 Reagents
CRO Services
Product recommended

All CD97 Reagents
Anti-CD97 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CD97 Antibody
Validated applications
ELISA,FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human CD97
No cross-reactivity in ELISA with Human CD55 / DAF
Immunogen
Recombinant Human CD97 protein (Catalog#11280-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD97 (rh CD97; Catalog#11280-H08H; NP_001775.2; Met 1-Gln 398).
Source
Monoclonal Rabbit IgG Clone #024
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD97 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD97 Antibody (Rabbit Monoclonal antibody) Images
Human CD97 Immunofluorescence(IF) 2964
Immunofluorescence staining of Human CD97 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD97 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cells membranes.
Human CD97 Flow Cytometry (FC) 5220
Flow cytometric analysis of Human CD97 expression on K562 cells. Cells were stained with purified anti-Human CD97, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-CD97 Antibody Alternative Names
Anti-CD97 Antibody;Anti-TM7LN1 Antibody
CD97 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium binding site. Muture CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isforms that are related to the calium binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.

Full Name
adhesion G protein-coupled receptor E5
References
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Gray JX, et al. (1996) CD97 is a processed, seven-transmembrane, heterodimeric receptor associated with inflammation. The journal of Immunology. 157 (12): 5438-47.
  • Hamann J,et al. (1998) Characterization of the CD55 (DAF)-binding site on the seven-span transmembrane receptor CD97.European Journal of Immunology. 28 (5): 1701-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD97 Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF,IP 11280-T54
    Anti-CD97 Antibody (FITC) Mouse Monoclonal FCM 11280-MM03-F
    Anti-CD97 Antibody Rabbit Polyclonal ELISA 11280-RP01
    Anti-CD97 Antibody (APC) Mouse Monoclonal FCM 11280-MM03-A
    Anti-CD97 Antibody (PE) Rabbit Monoclonal FCM 11280-R024-P
    Anti-CD97 Antibody Mouse Monoclonal ELISA,ELISA(Cap),ICC/IF,IF 11280-MM07
    Anti-CD97 Antibody Rabbit Polyclonal WB,ELISA,IP 11280-RP02
    Anti-CD97 Antibody (FITC) Rabbit Monoclonal FCM 11280-R024-F
    Anti-CD97 Antibody Mouse Monoclonal FCM,ICC/IF,IF 11280-MM03
    Anti-CD97 Antibody Mouse Monoclonal ELISA,ELISA(Det) 11280-MM05
    Anti-CD97 Antibody (APC) Rabbit Monoclonal FCM 11280-R024-A
    Anti-CD97 Antibody (PE) Mouse Monoclonal FCM 11280-MM03-P
    info info
    添加购物车成功! 添加购物车失败!