|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CD97 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg SW480 Whole Cell Lysate0.5 µL anti-CD97 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CD97 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 92 kDa
Observed band size: 92 kDa
Anti-CD97 rabbit polyclonal antibody at 1:500 dilution
Lane A: HL-60 Whole Cell Lysate
Lane B: Hela Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:92 kDa
Observed band size:92 kDa
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium binding site. Muture CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isforms that are related to the calium binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CD97 Antibody (FITC)||Mouse||Monoclonal||FCM||11280-MM03-F|
|Anti-CD97 Antibody (FITC)||Rabbit||Monoclonal||FCM||11280-R024-F|
|Anti-CD97 Antibody (APC)||Mouse||Monoclonal||FCM||11280-MM03-A|
|Anti-CD97 Antibody (PE)||Rabbit||Monoclonal||FCM||11280-R024-P|
|Anti-CD97 Antibody (APC)||Rabbit||Monoclonal||FCM||11280-R024-A|
|Anti-CD97 Antibody (PE)||Mouse||Monoclonal||FCM||11280-MM03-P|