Rat CD96 Gene cDNA Clone / ORF Clone

CD96 cDNA Clone Datasheet

CD96 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001025032.1
• cDNA Size: 1812
• Gene Synonym: Cd96
• Source: Rat

CD96 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus CD96 molecule DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 937 T/C，1540 G/A resulting in the amino acid Trp substitution by Arg and 1596 G/A not causing the amino acid variation.

CD96 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD96 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD96 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAAGAA AATGGACATA CTGTGTGGTT TACGCCATCA TCCAGATACA GTTTTTCAGG
61       GGAGCATGGG AAGAACTATT CAATGCTGGA GATAACAACG TGTATGCTCT GCCTGGTTCT
121      GATGTCAACT TGACCTGCCA AACAATGGAG AAAGACTTAA TGGTTCAGAT GCAGTGGTCC
181      AAGGTCACAG ATGAGATTGA TATGATTGTT GTTTATCATC CCCAATATGG CTTCCACTAT
241      ATGCAGGGGG TTGCCTGTGA GTCACGAGTG GCTGCGGTAG AAACTCTGAA GGATGCAACG
301      AAATGGACTC TGAACTTAAG GAATATCTCT TCCAGCCTCA GTGGAAAGTA TGAGTGCAGC
361      TTCACTATGT ATCCAACAGG CACCAAGACT ATAGTCTACA ACCTCATTGT GGAACCCTAT
421      ACACAAGATG AACACAACCG TACAATAGAA ATAGAGACAA ATCGGACTCT GGAAATACCA
481      TGCTTTCAAA ATACCTCCTC AGAAATTTCA CCTAGGTTCA CCTTTTCATG GTTGGTGGAG
541      AAAGATGGAG TGGAAGACGT TCTCTTCACC TACGATTACC ACGTCAGCAA TTCCACAGCA
601      TTTAAAGGCA GAGTTGGGCT GGGTGCAGAC TATGGACTCC GCCTCTCCCC AGTCCAAATC
661      CAAGATGATG GCAGGACATT CTCTTGCTTT CTGAGAATCA GTCCTCTCAA AGTCTGGAAG
721      ACATCCACCA CAGTCAAGGT TTTTGCTAAG CCAGAAATCC TCATGACTGT GGAAAACACC
781      ACCATGGATG TCTTAGGAGA GAGAGTATTT ACCTGCCTTC TGAAGAATGT TTTCCCCAAA
841      GCAAGTATTA CCTGGTTAAT AGATGGAAGA CTTTTTCAAG GCAATGAAGA AGGAATATAC
901      ATTACAAATG AAGAAAAAAA TAGCAGTAGC GGATTTCGGG AACTGAAGTC GGTTCTAACA
961      AGGATGCACA ATAGAACATC CCAATCAAAC AATATGACAG TTTGGTGTAT GGCTCTGTCG
1021     CCAGGCCCTG GAAATAAAAT GTGGAATACT TCATCACAAC CCATCACATT TTCCTTGGAT
1081     TCTGGGACTG CCCCAACAAA ACGTCTACCC AATGTGACAG GTTCTACCCT GGGTGCACAA
1141     ACCTTTCCAG ATGCCGAAGT ATCTCCTACA AGATATCTAG CTACATCTTC AATGACAATT
1201     GTAGATGAAA ATGTGTTGAC ACCAGACCCA ACTCCTCAAA CCAGCAATTC CAGCATGACT
1261     ACTAAAGATG TCAACTATTC CCAGCCCTCC AGTGGGACAG ATGCCAAGAA CTCCTCAAGA
1321     GCTGCTTCTT CTTCTGATGG TGGATCTCGG CCTTTTCCTT CCACCTCTCC TCCCAAATGG
1381     CTCTCACTGC CTCATACTTC CACTGGACCC CAGGAACCAG ACTCCGCAGT TTCATGGATA
1441     CCAACAGATG CGTACACTTC AGGCTCTTCA GATGCAAGCT TAACTTCTCA CGATGTCATC
1501     ATCCGTACAA CCAAAGAATT TCCAGATGTC CTCACAACTA CAAATGGAAC TACTAAAATT
1561     AAACATGGCC ACGTCACAGG AATCACAGTT AATAAACCCA GAGATGGAAT GTCCTGGCCT
1621     GTGGCTGTCG CGACTTTGCT ATTTTTCTGC ATACTGTTGT TTGGGCTTGG AGTGAGAAAA
1681     TGGTGTCAGT ATCAAAAGGA AATCATGGAA AGACCCCCAC CTTTCAAGCC ACCACCACCT
1741     CCCATCAAGT ACATGTGCAT TCAGGAACCC ACTGGACGTG GCATGCCTTG TCATGAGATG
1801     GAGGTCCTCT AA

 

Quality Control:cDNA sequencing results is available upon request.