Mouse CD96 Protein (His Tag)

CD96 antigen Protein Datasheet

CD96 Protein Product Information

• Synonym: 1700109I12Rik, Tactile
• Protein Construction: A DNA sequence encoding the mouse CD96 (Q3U0X8) extracellular domain (Met 1-Met 536) was expressed, fused with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

CD96 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized recombinant Mouse CD96 at 10 μg/ml (100 μl/well) can bind mouse PVR with a linear range of 6.4-160 ng/ml.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 22
• Molecular Mass: The secreted recombinant mouse CD96 comprises 526 amino acids and has a calculated molecular mass of 58 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD96 protein

CD96 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD96 Protein Description

T-cell surface protein tactile, also known as cell surface antigen CD96, T cell-activated increased late expression protein and CD96, is a single-pass type I? membrane protein which contains one?Ig-like C2-type (immunoglobulin-like) domain and two?Ig-like V-type (immunoglobulin-like) domains. CD96 is expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells. CD96 may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. It promotes NK cell-target adhesion by interacting with PVR present on target cells. CD96 may function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. Defects in CD96 are a cause of C syndrome (CSYN) which is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. Defects in CD96 are a cause of C-like syndrome (CLSYN). The C-like syndrome seems to be a severe form of the C syndrome.

References

1. Wang P.L., et al., 1992, J. Immunol. 148:2600-2608.
2. Fuchs A., et al., 2004, J. Immunol. 172:3994-3998.
3. Kaname T., et al., 2007, Am. J. Hum. Genet. 81:835-841.
4. Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.