CD96 Antibody (Antigen Affinity Purified)

CD96 Antibody ( Antigen Affinity Purified ) Datasheet

CD96 Antibody Product Information

• Immunogen: Recombinant Human CD96 protein (Catalog#11202-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD96 (rh CD96; Catalog#11202-H08H; P40200-2; Met 1-Met 503). CD96 specific IgG was purified by Human CD96 affinity chromatography.

CD96 Antibody Usage Guide

• Specificity: Human CD96
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD96 in WB. Using a DAB detection system, the detection limit for Human CD96 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD96. The detection limit for Human CD96 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD96 Antibody Background

T-cell surface protein tactile, also known as cell surface antigen CD96, T cell-activated increased late expression protein and CD96, is a single-pass type I membrane protein which contains oneIg-like C2-type (immunoglobulin-like) domain and twoIg-like V-type (immunoglobulin-like) domains. CD96 is expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells. CD96 may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. It promotes NK cell-target adhesion by interacting with PVR present on target cells. CD96 may function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. Defects in CD96 are a cause of C syndrome (CSYN) which is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. Defects in CD96 are a cause of C-like syndrome (CLSYN). The C-like syndrome seems to be a severe form of the C syndrome.

References

1. Wang P.L., et al., 1992, J. Immunol. 148: 2600-2608.
2. Fuchs A., et al., 2004, J. Immunol. 172: 3994-3998.
3. Kaname T., et al., 2007, Am. J. Hum. Genet. 81: 835-841.
4. Wollscheid B., et al., 2009, Nat. Biotechnol. 27: 378-386.