Mouse FASL Gene cDNA Clone / ORF Clone

FASL cDNA Clone Datasheet

FASL cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010177.4
• cDNA Size: 840
• Gene Synonym: gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1; Fasl
• Source: Mouse

FASL cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Fas ligand (TNF superfamily, member 6) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for three point mutations:550 A/G resulting in the amino acid Thr substitution by Ala, 653 A/G resulting in the amino acid Glu substitution by Gly, 567 G/A not causing the amino acid variation.

FASL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FASL cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FASL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGCAGC CCATGAATTA CCCATGTCCC CAGATCTTCT GGGTAGACAG CAGTGCCACT
61       TCATCTTGGG CTCCTCCAGG GTCAGTTTTT CCCTGTCCAT CTTGTGGGCC TAGAGGGCCG
121      GACCAAAGGA GACCGCCACC TCCACCACCA CCTGTGTCAC CACTACCACC GCCATCACAA
181      CCACTCCCAC TGCCGCCACT GACCCCTCTA AAGAAGAAGG ACCACAACAC AAATCTGTGG
241      CTACCGGTGG TATTTTTCAT GGTTCTGGTG GCTCTGGTTG GAATGGGATT AGGAATGTAT
301      CAGCTCTTCC ACCTGCAGAA GGAACTGGCA GAACTCCGTG AGTTCACCAA CCAAAGCCTT
361      AAAGTATCAT CTTTTGAAAA GCAAATAGCC AACCCCAGTA CACCCTCTGA AAAAAAAGAG
421      CCGAGGAGTG TGGCCCATTT AACAGGGAAC CCCCACTCAA GGTCCATCCC TCTGGAATGG
481      GAAGACACAT ATGGAACCGC TCTGATCTCT GGAGTGAAGT ATAAGAAAGG TGGCCTTGTG
541      ATCAACGAAG CTGGGTTGTA CTTCGTATAT TCCAAAGTAT ACTTCCGGGG TCAGTCTTGC
601      AACAACCAGC CCCTAAACCA CAAGGTCTAT ATGAGGAACT CTAAGTATCC TGGGGATCTG
661      GTGCTAATGG AGGAGAAGAG GTTGAACTAC TGCACTACTG GACAGATATG GGCCCACAGC
721      AGCTACCTGG GGGCAGTATT CAATCTTACC AGTGCTGACC ATTTATATGT CAACATATCT
781      CAACTCTCTC TGATCAATTT TGAGGAATCT AAGACCTTTT TCGGCTTGTA TAAGCTTTAA

 

Quality Control:cDNA sequencing results is available upon request.