CD95 / APO-1 / TNFRSF6 / FAS Antibody

CD95 / APO-1 / TNFRSF6 / FAS Antibody Datasheet

CD95 / APO-1 / TNFRSF6 / FAS Antibody Product Information

• Immunogen: Recombinant Human CD95 protein (Catalog#10217-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 2F2B2C5
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD95 / APO-1 / TNFRSF6 / FAS (rh CD95 / APO-1 / TNFRSF6 / FAS; Catalog#10217-H08H; NP_000034.1; Met 1-Asn 173). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD95 / APO-1 / TNFRSF6 / FAS Antibody Usage Guide

• Specificity: Human CD95 / APO-1 / TNFRSF6 / FAS
• No cross-reactivity in ELISA with

  Human BAFF

  Human TNFRSF10A

  Human TNFa

  Human TNFSF11

  Human cell lysate (293 cell line)
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human FAS in WB. Using a DAB detection system, the detection limit for Human FAS is approximately 20 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human FAS. The detection limit for Human FAS is approximately 0.16 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD95 / APO-1 / TNFRSF6 / FAS Antibody Background

Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the tumor necrosis factor (TNF) / nerve growth factor (NGF) receptor superfamily. Fas is highly expressed on the surface of a number of cell types including epithelial cells, hepatocytes, activated mature lymphocytes and other tumor cells. This receptor contains a death domain and has been shown to play a central role in the physiological regulation of programmed cell death (PCD, apoptosis) upon ligation of Fas ligand (Fas-L). This Fas receptor has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. Multiple Fas isoforms are derived from alternative spliced transcript variants, and among which, a soluble form lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoforms.

References

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