Mouse THY1 Gene cDNA Clone / ORF Clone

THY1 cDNA Clone Datasheet

THY1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009382.3
• cDNA Size: 489
• Gene Synonym: T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2; Thy1
• Source: Mouse

THY1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus thymus cell antigen 1, theta DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

THY1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

THY1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

THY1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACCCAG CCATCAGCGT CGCTCTCCTG CTCTCAGTCT TGCAGGTGTC CCGAGGGCAG
61       AAGGTGACCA GCCTGACAGC CTGCCTGGTG AACCAAAACC TTCGCCTGGA CTGCCGCCAT
121      GAGAATAACA CCAAGGATAA CTCCATCCAG CATGAGTTCA GCCTGACCCG AGAGAAGAGG
181      AAGCACGTGC TCTCAGGCAC CCTTGGGATA CCCGAGCACA CGTACCGCTC CCGCGTCACC
241      CTCTCCAACC AGCCCTATAT CAAGGTCCTT ACCCTAGCCA ACTTCACCAC CAAGGATGAG
301      GGCGACTACT TTTGTGAGCT TCAAGTCTCG GGCGCGAATC CCATGAGCTC CAATAAAAGT
361      ATCAGTGTGT ATAGAGACAA GCTGGTCAAG TGTGGCGGCA TAAGCCTGCT GGTTCAGAAC
421      ACATCCTGGA TGCTGCTGCT GCTGCTTTCC CTCTCCCTCC TCCAAGCCCT GGACTTCATT
481      TCTCTGTGA

 

Quality Control:cDNA sequencing results is available upon request.