Mouse THY1 / CD90 Protein (His Tag)

Mouse Thy-1 Membrane Glycoprotein ( THY1 / CD90 ) Protein

THY1 / CD90 Protein Product Information

• Synonym: Thy1, CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse THY1 ( NP_033408.1 ) without the propeptide ( Met 1 - Cys 131 ) was expressed, with a polyhistidine tag at the C-terminus
• Source: Mouse
• Expression Host: Human Cells

THY1 / CD90 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 20
• Molecular Mass: The recombinant mouse THY1 consists of 123 amino acids and has a predicted molecular mass of 14.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm THY1 is approximately 20-27 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: THY1 protein

THY1 / CD90 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

THY1 / CD90 Protein Description

Mouse Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. THY1 / CD90, a member of the immunoglobulin superfamily of adhesion molecules with constitutive expression on fibroblast cells. THY1 / CD90 is a cell surface glycoprotein originally identified on mouse thymocytes. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. THY1 / CD90 is a membrane glycoprotein expressed in thymus, retinal ganglionic cells, and several types of stem cells. It was observed that THY1 / CD90 cell surface expression decreased significantly during the course of infection. In adult rats, expression of THY1 / CD90 on the B cell lineage is confined to immature B cells. The expression of THY1 / CD90 on neonatal MZ-B cells may have implications for their responsiveness to polysaccharide (T cell-independent type 2) antigens. THY1 / CD90 is modulated following infection with Human cytomegalovirus (HCMV). Neuroblastoma (NBL) is the most common solid tumor in children. THY1 / CD90 seemed to be a marker with a specific prognostic value in NBL patients. THY1 / CD90 may also play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

References

1. Dammers, PM. et al., 2002, Dev Immunol  9 (4):187-95.
2. Leis,M. et al., 2004, J Gen Virol 85 (Pt 7):1995-2000.
3. Wiesmann,A. et al., 2006, Head Face Med 2 :8.
4. Fiegel, HC. et al., 2008, Pediatr Surg Int  24 (1):101-5.
5. Kisselbach,L. et al., 2009, Cytotechnology. 59 (1): 31-44.