pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Rat CD9 ORF mammalian expression plasmid, C-GFPSpark tag||RG80778-ACG|
|Rat CD9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80778-ACR|
|Rat CD9 ORF mammalian expression plasmid, C-Flag tag||RG80778-CF|
|Rat CD9 ORF mammalian expression plasmid, C-His tag||RG80778-CH|
|Rat CD9 ORF mammalian expression plasmid, C-Myc tag||RG80778-CM|
|Rat CD9 ORF mammalian expression plasmid, C-HA tag||RG80778-CY|
|Rat CD9 ORF mammalian expression plasmid, N-Flag tag||RG80778-NF|
|Rat CD9 ORF mammalian expression plasmid, N-His tag||RG80778-NH|
|Rat CD9 ORF mammalian expression plasmid, N-Myc tag||RG80778-NM|
|Rat CD9 ORF mammalian expression plasmid, N-HA tag||RG80778-NY|
|Rat CD9 Gene cDNA clone plasmid||RG80778-U|
|Rat CD9 natural ORF mammalian expression plasmid||RG80778-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD9 is a member of the transmembrane 4 superfamily, which is also known as the tetraspanin family. CD9 is a cell surface glycoprotein with 4 hydrophobic domains that is described to complex with integrins and other transmembrane 4 superfamily members. It is found expressed on the surface of the exosomes. The protein takes part in cellular signal transduction events and thus play a role in the regulation of cell development and activation, growth and motility. Besides, CD9 seems to be a key role in the egg-sperm fusion during the mammalian fertilization processes. CD9 is found on the membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.