CD9 Antibody ( PE )

CD9 Antibody Datasheet

CD9 Antibody Product Information

• Immunogen: Recombinant Human CD9 protein (Catalog#11029-H08H)
• Reagents: PE-conjugated mouse monoclonal antibody
• Clone ID: 01
• Ig Type: Mouse IgG1
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD9 (rh CD9; Catalog#11029-H08H; NP_001760.1; Ser 112-Ile 195) and conjugated with PE under optimum conditions, the unreacted PE was removed.

CD9 Antibody Usage Guide

• Specificity: Human CD9
• Flow Cytometry:
  

  Profile of anti-CD9 reactivity on HeLa cells analyzed by flow cytometry.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD9 Antibody Background

CD9 antigen, also known as 5H9 antigen, cell growth-inhibiting gene 2 protein, leukocyte antigen MIC3, motility-related protein, tetraspanin-29, CD9 and MIC3, is a multi-pass membrane protein which belongs to thetetraspanin (TM4SF) family. It is a cell surface marker expressed on myeloid and nonmyeloid as well as on neoplastic cells. CD9 forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Numerous functions have been attributed to CD9 and other members of the transmembrane 4 (TM4) superfamily. CD9 is thought to be involved in cell proliferation, differentiation, motility and survival. It may also function as part of toxin and virus receptor complexes. CD9 is involved in platelet activation and aggregation. It regulates paranodal junction formation and is involved in cell adhesion, cell motility and tumor metastasis. CD9 is required for sperm-egg fusion. CD9 associates with CR2/CD21 and with PTGFRN/CD9P1. It interacts directly with IGSF8.

References

1. Hayashi,S. et al., 2000, Leuk Lymphoma38 (3-4): 265-70.
2. Charrin S.,et al., 2001, J. Biol. Chem. 276: 14329-37.
3. Stipp C.S., et al., 2001, J. Biol. Chem. 276: 40545-54.
4. Higginbottom A., et al., 2003, Biochem. Biophys. Res. Commun. 311: 208-14.
5. Kovalenko O.V., et al., 2004, Biochem. J. 377: 407-17.