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Ferret CD8a / CD8 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| FG60001-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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CD8a / CD8 cDNA Clone Datasheet
CD8a / CD8 cDNA Clone Product Information
| Gene Bank Ref.ID: | EF492056.1 |
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cDNA Size: |
739 |
| Gene Synonym: |
CD8 |
| Source: | Ferret |
CD8a / CD8 cDNA Clone Description
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cDNA Description: |
ORF Clone of Ferret CD8 antigen DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref.ID sequence. |
CD8a / CD8 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
CD8a / CD8 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
CD8a / CD8 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGCCTCTC GGGTGACCCC CCTGCTCCTG CCGCTGGCCC TGCTGCTCCA TGCCGTCGCG 61 GCCGCCGGGC CGAGCCAGTT CCGCTTGTCG CCGGCGAAGG TGGTCGGACA GCTGGGAGAG 121 AAAGTGGAGC TGCAGTGCGA GGTGCTGCTG CCCTCCGCGG CGCCGGGCTG TTCCTGGCTC 181 CTCCAGAAGA ACGAACCTGC CGCCCGCCCC GTCTTCCTCA TGTACCTCTC CCAAACCCGG 241 ACCAAGTTGG CCGACGGACT GGACTCCGAA CAGATTTCGG GCAAGAAGAT CCGTGACACC 301 CTCTACAGCC TCACCCTGCG CCGCTTCCGC AAGGAGGACG AAGGCTACTA TTTCTGCTCC 361 GTCCTGAGCA ATTCGGTGCT GTACTTCAGC TCCTTCGTGC CGGTCTTCCT GCCAGTCAAG 421 CCCACCCCTA CGCCCGCGCC GCGATTACCC ACGAGGGCGC CCACCAACAC GTCGCAGCCG 481 GTGTCTCAGC GCCCCGGGAT CTGCCGGCCG CCGGCGGGCA AGCCAGTGGA AAAAGGTGTG 541 CTTGGCTTTG CCTGTGAAAT CTACATCTGG GCACCGCTGG CTGGGACCTG CGCGGTCCTT 601 CTACTGTCAC TGGTCATCAC CGTCATCTGC AACCATAGGA ACAGAAGACG TGTTTGCAAA 661 TGTCCCAGGC CAATGCCCAG ACAAGGAGGA CCAGGAGGCA AGCCCAGCCC TTCAGAGAAA 721 TATGTCTAAC ATGGAGATG
Quality Control:cDNA sequencing results is available upon request.
