|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) t-cell surface glycoprotein CD8 beta chain-like DNA.|
|Identical with NM_001145533.1 [ Macaca mulatta (Rhesus monkey) ]: 481-483 AGC/GCG resulting in the amino acid Ser substitution by Ala and 480 T/A not causing the amino acid variation. Please check the sequence information before order.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Cynomolgus monkey CD8B Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||CG90178-G-F|
|Cynomolgus monkey CD8B Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||CG90178-G-H|
|Cynomolgus monkey CD8B Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||CG90178-G-M|
|Cynomolgus monkey CD8B Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||CG90178-G-N|
|Cynomolgus monkey CD8B Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||CG90178-G-Y|
|Product name||Product name|
CD8B (CD8b molecule), also known as P37 and LEU2, contains 1 Ig-like V-type (immunoglobulin-like) domain. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. P37 gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. CD8 is thought to play a role in the process of T-cell mediated killing.