Rat CD8A Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80285-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD8A cDNA Clone Datasheet

CD8A cDNA Clone Product Information

Gene Bank Ref.ID:	NM_031538.2
cDNA Size:	711
Gene Synonym:	Cd8a
Source:	Rat

CD8A cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus CD8a molecule DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CD8A cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD8A cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD8A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCTCAC GGGTGATCTG CTTTCTGTCG CTGAACCTGC TACTGCTGGA TGTTATCACT
61       AGGCTCCAGG TTTCCGGACA GTTACAGTTG TCACCAAAGA AAGTGGACGC TGAAATTGGC
121      CAGGAGGTGA AGCTAACATG CGAAGTGCTG CGGGACACTT CGCAAGGATG CTCTTGGCTC
181      TTCCGGAACT CCAGCTCCGA ACTCCTCCAG CCCACCTTCA TCATCTATGT ATCTTCATCC
241      CGGAGCAAGC TGAACGATAT ACTGGATCCG AATCTGTTCT CTGCCCGGAA GGAAAACAAC
301      AAATACATCC TCACCCTGAG CAAGTTCAGC ACTAAAAACC AAGGCTACTA TTTCTGCTCA
361      ATCACCAGCA ACTCGGTGAT GTACTTCAGT CCTCTGGTGC CGGTGTTTCA GAAAGTGAAC
421      TCTATTATCA CCAAGCCGGT GACGCGAGCT CCCACACCAG TGCCTCCTCC TACAGGGACA
481      CCCCGGCCCC TACGACCAGA AGCTTGCCGA CCCGGGGCGA GTGGCTCAGT GGAGGGAATG
541      GGATTGGGCT TCGCCTGCGA TATTTACATC TGGGCACCCT TGGCCGGAAT CTGCGCGGTT
601      CTTCTGCTGT CCCTGGTCAT CACTCTCATC TGCTGCCACA GGAACCGAAG GCGTGTTTGC
661      AAATGTCCCA GGCCCCTTGT CAAGCCCAGA CCTTCAGAGA AATTCGTGTA A

Quality Control:cDNA sequencing results is available upon request.