Rat CD89/FCAR Gene cDNA Clone / ORF Clone

FCAR cDNA Clone Datasheet

FCAR cDNA Clone Product Information

• Gene Bank Ref.ID: NM_201992.1
• cDNA Size: 843
• Gene Synonym: CD89; Fcar
• Source: Rat

FCAR cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus IgA Fc receptor DNA.
• Vector: Cloning Vector: pMD18-T Simple Vector.

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 606 A/T not causing the amino acid variation.

FCAR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCAR cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FCAR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCCTC AGGACAACAC CTTCCTATGT CTTGTGATCT GCCTGGGTCA GACAATTTGG
61       GCACAAGAAG ATTTTCCCAT TCCCATCATA TCCACTGCAA CAAATCCTAT GGTTTCCTGG
121      AATGAGTCTG TGAGGATCCT GTGCAGGGGA ACACCGGAAG CCTTCCTGTA TCAGTTGTCC
181      CTGATGAAAA ACTCCACACA AACAGTTATA GAGAAGAAAC TAGGATTTCA AAAAGAGGCT
241      GAATTCATTA TCAACCACAT GAATTCAACA CTGGCAGGAT GTTATCAGTG TCAGTATAGG
301      AAGAAGAACC ACTGGTCAGA GCAGAGTAAA CCCCTGAAGC TAGTGGTGAC AGGCTTGTAT
361      GACAAACCTG TCCTCTCCAC AGATCAAAGC CTTGTGTTGT TACCAGGAGA GAACGTTTCC
421      TTCAAGTGTA GTTCAGCCCA CAACCTGTTC AACAGATTTT CACTGGCCAA AGAGGGAGAA
481      GCTTCCTTGC CATTGCACCA ACATAAGGAG TCTCAAGGCA ACTTCCACCT GGGTCCTGTG
541      AACGCTGACT TCATTGGAAA ATACAGATGC TACGGCTGGC ACAACAACAG CCCCTATGTG
601      TGGTCTGCCC CCAGTGATGC CCTGGAGCTC ATTGTCTCAG TTACATCTCT AGACTCCGAG
661      AAGCATGATT ACGTGATGGA GAATTCAATC CGGATAGGTA TGGCTGGGCT GGTCCTTGTC
721      ATACTCTTGG TGATAGTATC TGAAGATTGG TACAGCCACA GGATCTCACA CAAGGAATGC
781      TGTCAGGAAC GGGCTGCAAA GAGATGGAGC AAACAGGGTG TCAGACCTAG TGACTGTAGA
841      TAA

 

Quality Control:cDNA sequencing results is available upon request.