Rat FCAR / CD89 Protein (His Tag)

Rat Immunoglobulin Alpha Fc Receptor / CD89 / FCAR Protein

Rat CD89 / FCAR Protein Product Information

• Synonym: CD89
• Protein Construction: A DNA sequence encoding the rat FCAR ( NP_973721.1 ) extracellular domain ( Met 1 - Asn 228 ) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Rat
• Expression Host: Human Cells

Rat CD89 / FCAR Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 23
• Molecular Mass: The recombinant rat FCAR comprises 217 amino acids and predicts a molecular mass of 24.8 kDa. The apparent molecular mass of the rat FCAR is approximately 35-40 kDa in SDS-PAGE under reducing conditions due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD89 protein

Rat CD89 / FCAR Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Rat CD89 / FCAR Protein Description

Rat FCAR, also called FcαRI or CD89, is a type I transmembrane receptor for Fc region of IgA which is the most abundant immunoglobulin in mucosal areas but is only the second most common antibody isotype in serum. This receptor is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, especially phagocytes located in mucosal areas. Upon ligand IgA binding, FCAR / CD89 associates with the FcR γ signaling molecule bearing the immunoreceptor tyrosine-based activation motif (ITAM) through a unique charge-based mechanism and triggers multiple cell-mediated immune responses. It has been reported that Fc RI is a dual-function receptor that can mediate both inflammatory and anti-inflammatory responses depending on the type of interaction with its ligand. Sustained aggregation of FCAR results in activation of target-cell functions such as antigen presentation and cytokine release. In contrast, Monomeric targeting with serum IgA or with a variety of anti-FcαRI Fab fragments triggers an inhibitory response and additionally induces apoptosis. FCAR / CD89 play an fundamental role in preventing tumor development and growth, as well as in controlling inflammation.

References

1. Maliszewski, C.R. et al., 1990, J. Exp. Med. 172: 1665-1672.
2. Launay, P. et al., 1999, J. Biol. Chem. 274: 7216-7225.
3. Monteiro, R.C. et al., 2003, Annu. Rev. Immunol. 21: 177-204.
4. Otten, M.A. et al., 2005, J. Immunol. 174: 5472-5480.
5. Pasquier, B. et al., 2005, Immunity. 22:31-42.
6. Kanamaru, Y. et al., 2007, Blood. 109: 203-211.