CD89 / FCAR Antibody (Rabbit Monoclonal Antibody)

Immunoglobulin alpha Fc Receptor ( CD89 / FCAR ) Antibody

CD89 / FCAR Antibody Product Information

• Immunogen: Recombinant human CD89 protein ( Catalog#10414-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 6
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD89 / FCAR ( rh CD89 ; Catalog#10414-H08H ; NP_001991.1 ; Met 1- Asn 227 ).

CD89 / FCAR Antibody Usage Guide

• Specificity: Human CD89 / FCAR
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CD89 in WB. Using a DAB detection system, the detection limit for human CD89 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human CD89. The detection limit for human CD89 is 0.0049 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD89 / FCAR Antibody Background

FCAR, also called FcαRI or CD89, is a type I  transmembrane receptor for Fc region of IgA which is the most abundant immunoglobulin in mucosal areas but is only the second most common antibody isotype in serum. This receptor is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, especially phagocytes located in mucosal areas. Upon ligand IgA binding, CD89 associates with the FcR γ signaling molecule bearing the immunoreceptor tyrosine-based activation motif (ITAM) through a unique charge-based mechanism and triggers multiple cell-mediated immune responses. It has been reported that Fc RI is a dual-function receptor that can mediate both inflammatory and anti-inflammatory responses depending on the type of interaction with its ligand. Sustained aggregation of FCAR results in activation of target-cell functions such as antigen presentation and cytokine release. In contrast, Monomeric targeting with serum IgA or with a variety of anti-CD89 Fab fragments triggers an inhibitory response and additionally induces apoptosis. CD89 play an fundamental role in preventing tumor development and growth, as well as in controlling inflammation.

References

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2. Launay, P. et al., 1999, J. Biol. Chem. 274: 7216-25.
3. Monteiro, RC. et al., 2003, Annu. Rev. Immunol. 21: 177-204.
4. Otten, MA. et al., 2005, J. Immunol. 174: 5472-80.
5. Pasquier, B. et al., 2005, Immunity. 22: 31-42.
6. Kanamaru, Y. et al., 2007, Blood. 109: 203-11.